CAJ | 학술논문

稻瘟病抗病基因Pi-k h是对稻瘟病菌具有广谱抗性的一个重要基因.根据 Pi-k h 感病基因和抗病基因序列存在143bp 插入的特征，设计了 InDel 标记 FM143.Tetep具有抗病基因Pi-kh ，分子标记 FM143 PCR 扩增带型为非插入带型，而不携带Pi-kh的材料的带型为插入带型.利用该标记对Tetep与宁9108的 F2群体进行分析，结果表明该标记能准确区分Pi-kh、Pi-kh纯合及杂合基因型，标记基因型符合1∶2∶1比例，没有发生偏分离现象.利用该标记对65份江苏省历年来大面积推广的粳稻品种和64份粳稻品系进行检测，筛选出具有非插入带型的粳稻品种19份、粳稻品系22份.进一步对江苏省粳稻品种中Pi-k h位点进行测序，发现江苏粳稻品种(系)Pi-k h基因座位与 Tetep 和已经登录的Pi-k h序列相比在102 bp处存在一个单碱基的变异(由 G突变为C)，使得谷氨酸变异为天门冬氨酸，且该变异高度保守.将江苏省粳稻品种中发现的Pi-k h位点的等位基因暂定名为Pi-kh JSJ .
도온병항병기인Pi-k h시대도온병균구유엄보항성적일개중요기인.근거 Pi-k h 감병기인화항병기인서렬존재143bp 삽입적특정，설계료 InDel 표기 FM143.Tetep구유항병기인Pi-kh ，분자표기 FM143 PCR 확증대형위비삽입대형，이불휴대Pi-kh적재료적대형위삽입대형.이용해표기대Tetep여저9108적 F2군체진행분석，결과표명해표기능준학구분Pi-kh、Pi-kh순합급잡합기인형，표기기인형부합1∶2∶1비례，몰유발생편분리현상.이용해표기대65빈강소성력년래대면적추엄적갱도품충화64빈갱도품계진행검측，사선출구유비삽입대형적갱도품충19빈、갱도품계22빈.진일보대강소성갱도품충중Pi-k h위점진행측서，발현강소갱도품충(계)Pi-k h기인좌위여 Tetep 화이경등록적Pi-k h서렬상비재102 bp처존재일개단감기적변이(유 G돌변위C)，사득곡안산변이위천문동안산，차해변이고도보수.장강소성갱도품충중발현적Pi-k h위점적등위기인잠정명위Pi-kh JSJ .
Pi-kh is a vital non race-specific resistance gene for rice blast.In this study,one InDel marker,FM143 was developed based on the 143 bp insertion between the susceptible and resistant Pi-kh alleles.Previous studies showed that Tetep carried resistant Pi-kh allele and had the none-insertion genotype for PCR by FM143.F2 population,derived from Tetep and Ning 9108,were detected by marker FM143 and the result indicated that FM143 could effectively identify Pi-kh/Pi-kh ,Pi-kh/pi-kh and pi-kh/pi-kh genotypes with the expected segregation ratio 1∶2∶1.Sixty-five japonica varieties widely grown in Jiangsu Province over years and sixty-four japonica lines were genotyped by FM143. Among them,19 japonica varieties and 22 japonica lines showed none-insertion genotype.Further sequencing at the target Pi-kh locus for these none-insertion genotype showed that there was one highly conserved single-base mutation (G→C)at 102 bp location compared with the whole target gene sequence in Tetep and public Pi-kh GeneBank leadingPi-khJSJ.