CAJ | 학술논문

通过测定蜡质含量、硅化细胞、硅含量以及相关防御酶和防卫基因诱导表达特征,对抗纹枯病品系 YSBR1的抗性机制进行了初步研究。结果显示,叶片和叶鞘的蜡质含量在不同抗、感品种间差异不显著；叶片上、下表面的硅化细胞排列密度在各品种间总体差异不明显,各品种叶鞘内侧均未发现硅化细胞；纹枯菌接种后 YSBR1叶片下表面和叶鞘内表面硅元素含量均显著或极显著上升,中抗品种 Jasmine 85叶鞘内表面硅含量亦极显著上升,感病品种 Lemont 不同部位的硅含量在处理之间差异均不显著；纹枯病菌接种后 YSBR1中的苯丙氨酸解氨酶和多酚氧化酶活性的诱导表达及活性高峰值总体上快于或高于其它供试品种；接种后12 h,防卫基因 OsPR4的表达量在 YSBR1中急速上升且明显高于 Jasmine 85和 Lemont, OsPR10在特青中为诱导表达,但在 YSBR1和 Lemont 中为组成型强表达。综上,YSBR1对纹枯病的高水平抗性与其防卫反应的快速启动有关。
통과측정사질함량、규화세포、규함량이급상관방어매화방위기인유도표체특정,대항문고병품계 YSBR1적항성궤제진행료초보연구。결과현시,협편화협초적사질함량재불동항、감품충간차이불현저；협편상、하표면적규화세포배렬밀도재각품충간총체차이불명현,각품충협초내측균미발현규화세포；문고균접충후 YSBR1협편하표면화협초내표면규원소함량균현저혹겁현저상승,중항품충 Jasmine 85협초내표면규함량역겁현저상승,감병품충 Lemont 불동부위적규함량재처리지간차이균불현저；문고병균접충후 YSBR1중적분병안산해안매화다분양화매활성적유도표체급활성고봉치총체상쾌우혹고우기타공시품충；접충후12 h,방위기인 OsPR4적표체량재 YSBR1중급속상승차명현고우 Jasmine 85화 Lemont, OsPR10재특청중위유도표체,단재 YSBR1화 Lemont 중위조성형강표체。종상,YSBR1대문고병적고수평항성여기방위반응적쾌속계동유관。
The preliminary mechanism of the new rice line YSBR1 with high resistance to sheath blight disease(SB)was investigated through analyzing the content of wax and silicon,the arrangement of silicon cell,the activity of the well-known defense enzymes,and the expression pattern of some pathogenesis-related (PR)genes in response to SB fungus infection.The wax contents of leaves and leaf sheaths were not significantly different among varieties with various resistance levels to SB disease including YSBR1 .The arrangement of silicon cells in the upper and lower surfaces of leaf blades was similar among varieties,and no silicon cell was found in the inner surfaces of leaf sheaths.In response to SB fungus infection,the contents of silicon in the lower surfaces of leaf blades and the inner surfaces of leaf sheaths in YSBR1 were significantly induced,as well as in inner surfaces of leaf sheaths of Jasmine 85 with moderate resistance to SB.However,it was not found in all tissues tested in susceptible Lemont.In YSBR1 ,the activities of phenylalanine ammonia lyase(PAL )and polyphenol oxidase(PPO)were induced faster and significantly higher by SB fungus than those in other varieties.The expression level of OsPR4 in YSBR1 was upregulated rapidly and obviously higher than those in Jasmine 85 and Lemont 12 h post inoculation.The OsPR10 gene was found constitutively expressed in YSBR1 and Lemont,but induced expression in Jasmine 85 by SB fungus infection.Taken together,our results suggest that the defense system of YSBR1 is rapidly activated upon SB fungus infection,which is responsible for its high resistance to SB.