CAJ | 학술논문

目的：对从狼毒中分离得到的4种提取物进行体外抗肿瘤活性筛选。方法采用四甲基偶氮唑盐法研究狼毒提取物对SMMC-7721、HeLa、HepG2、H4604种人癌细胞株增殖的影响。结果狼毒提取物可以不同程度地抑制4种人癌细胞株的增殖。其中炮制品石油醚部位抑制作用最强，对 SMMC-7721、HepG2、H460细胞株增殖抑制率分别为81．08％，80．39％和89.81％；炮制品乙酸乙酯部位的抑制率分别为79．06％，78．36％和67．33％。结论狼毒炮制品石油醚部位和乙酸乙酯部位是狼毒抗肿瘤作用的主要活性部位，值得进一步深入探讨和研究。
목적：대종랑독중분리득도적4충제취물진행체외항종류활성사선。방법채용사갑기우담서염법연구랑독제취물대SMMC-7721、HeLa、HepG2、H4604충인암세포주증식적영향。결과랑독제취물가이불동정도지억제4충인암세포주적증식。기중포제품석유미부위억제작용최강，대 SMMC-7721、HepG2、H460세포주증식억제솔분별위81．08％，80．39％화89.81％；포제품을산을지부위적억제솔분별위79．06％，78．36％화67．33％。결론랑독포제품석유미부위화을산을지부위시랑독항종류작용적주요활성부위，치득진일보심입탐토화연구。
Objective To screen the main antitumor activity of four extracts of Euphorbia ebracteolata in vitro .Methods The four extracts obtained from E .ebracteolatawere evaluated for their cytotoxic activity against SMMC-7721 ,HeLa ,HepG2 and H460 by using MTT assay .Results Extracts of E .ebracteolatashowed potential antiproliferative activity against the four cell lines applied in different degree .Among the four extracts ,petroleum ether extract of processed E .ebracteolata showed the highest activity with inhibition ratio(IR) of 81 .08% ,80 .39% and 89 .81% against SMMC-7721 ,HepG2 and H460 ,respectively .And IR of ethyl ace-tate extracts of processed E .ebracteolata were 79 .06% ,78 .36% and 67 .33% ,respectively .Conclusion Petroleum ether extracts and ethyl acetate extracts are proved to be the effective anticancer agents in vitro .