CAJ | 학술논문

目的：建立复合多维元素片中泛酸的含量测定方法。方法高效液相色谱法，C18色谱柱（250 mm ×4．6 mm ，5μm ）；流动相：乙腈-1 mL · L -1磷酸水溶液（5∶95）；流速：1．0 mL · min-1；柱温：25℃；检测波长：200 nm。结果泛酸质量浓度在1．32～132．32μg · mL -1范围内具有良好的线性关系，相关系数 r＝0．9999，平均回收率为99．3％，RSD为1．4％（n＝12）。结论该方法快速简便，结果准确，适用于多维元素片中泛酸含量测定。
목적：건립복합다유원소편중범산적함량측정방법。방법고효액상색보법，C18색보주（250 mm ×4．6 mm ，5μm ）；류동상：을정-1 mL · L -1린산수용액（5∶95）；류속：1．0 mL · min-1；주온：25℃；검측파장：200 nm。결과범산질량농도재1．32～132．32μg · mL -1범위내구유량호적선성관계，상관계수 r＝0．9999，평균회수솔위99．3％，RSD위1．4％（n＝12）。결론해방법쾌속간편，결과준학，괄용우다유원소편중범산함량측정。
Objective To develop a method for determining the content of pantothenic acid in Vitamins with Minerals Tablets .Meth-ods The HPLC was carried out on a C18 (25 mm × 4 .6 mm ,5 μm) column ;the mobile phase consisted of acetonitrile-1 mL · L -1 phosphoric acid solution(5∶95)with flow rate of 1 .0 mL · min-1 ;the column temperature was set at 25℃ ;the detection wave-length was set at 200 nm .Results The linearity of the method was ranged from 1 .32 to 132 .32 μg · mL -1 (r=0 .999 9) ,and the average recovery rate was 99 .3% ,with RSD 1 .4% (n=12) .Conclusions This method is rapid ,simple ,accurate ,and applicable to the determination of pantothenic acid in Vitamins with Minerals Tablets .