西北药学杂志
西北藥學雜誌
서북약학잡지
2014年
2期
138-140
,共3页
韦华梅%石磊%党学良%刘新友%张琰
韋華梅%石磊%黨學良%劉新友%張琰
위화매%석뢰%당학량%류신우%장염
甘草酸%苦参碱%血药质量浓度%HPLC
甘草痠%苦參堿%血藥質量濃度%HPLC
감초산%고삼감%혈약질량농도%HPLC
glycyrrhizic acid%matrine%plasma concentration%HPLC
目的:建立以HPLC法同时测定人血浆中甘草酸和苦参碱质量浓度的方法。方法Ultimate AQ-C18柱(250mm×4.6mm,5μm),柱温为室温(25℃),流动相为乙腈-三乙胺调pH至7.5的0.05mol·L-1磷酸二氢钾(22.5∶77.5),流速为1.0mL·min-1,检测波长为220nm(检测苦参碱)和250nm(检测甘草酸),进样量50μL。结果甘草酸单铵盐质量浓度在2.0~200.0μg·mL-1范围内线性良好,苦参碱质量浓度在2.0~200.0μg·mL-1范围内线性良好。平均回收率均大于95%,日内、日间RSD均小于4%。结论该方法简便、快速、灵敏、可靠,可用于人血浆中甘草酸和苦参碱质量浓度的监测。
目的:建立以HPLC法同時測定人血漿中甘草痠和苦參堿質量濃度的方法。方法Ultimate AQ-C18柱(250mm×4.6mm,5μm),柱溫為室溫(25℃),流動相為乙腈-三乙胺調pH至7.5的0.05mol·L-1燐痠二氫鉀(22.5∶77.5),流速為1.0mL·min-1,檢測波長為220nm(檢測苦參堿)和250nm(檢測甘草痠),進樣量50μL。結果甘草痠單銨鹽質量濃度在2.0~200.0μg·mL-1範圍內線性良好,苦參堿質量濃度在2.0~200.0μg·mL-1範圍內線性良好。平均迴收率均大于95%,日內、日間RSD均小于4%。結論該方法簡便、快速、靈敏、可靠,可用于人血漿中甘草痠和苦參堿質量濃度的鑑測。
목적:건립이HPLC법동시측정인혈장중감초산화고삼감질량농도적방법。방법Ultimate AQ-C18주(250mm×4.6mm,5μm),주온위실온(25℃),류동상위을정-삼을알조pH지7.5적0.05mol·L-1린산이경갑(22.5∶77.5),류속위1.0mL·min-1,검측파장위220nm(검측고삼감)화250nm(검측감초산),진양량50μL。결과감초산단안염질량농도재2.0~200.0μg·mL-1범위내선성량호,고삼감질량농도재2.0~200.0μg·mL-1범위내선성량호。평균회수솔균대우95%,일내、일간RSD균소우4%。결론해방법간편、쾌속、령민、가고,가용우인혈장중감초산화고삼감질량농도적감측。
Objective To establish an HPLC methods for the simultaneous determination of human plasma concentration of glycyr-rhizic acid and matrine .Methods Ultimate AQ-C18 (250 mm × 4 .6 mm ,5 μm) was chosen as the chromatographic column .The column temperature was at room temperature(25 ℃) .The mobile phase was composed of acetonitrile and 0 .05 mol · L -1 aqueous phosphoric acid(22 .5∶77 .5 ,adjusted to pH 7 .5 with triethylamine) .The flow rate was set at 1 .0 mL · min-1 .The detection wavelengths were set at 220 and 250 nm .The injection volume was 50μL .Results The linear ranges of glycyrrhizic acid ammoni-um salt and matrine were 2 .0-200 .0 μg · mL -1 and 2 .0-200 .0 μg · mL -1 ,respectively .The average recovery was above 95% ;both the intraday RSD and the interday RSD were less than 4% .Conclusions The method is simple ,rapid ,sensitive and reliable , and could be used for the simultaneous determination of human plasma concentration of glycyrrhizic acid and matrine .