CAJ | 학술논문

目的：建立以HPLC法同时测定人血浆中甘草酸和苦参碱质量浓度的方法。方法Ultimate AQ-C18柱（250mm×4.6mm，5μm），柱温为室温（25℃），流动相为乙腈-三乙胺调pH至7．5的0．05mol·L-1磷酸二氢钾（22．5∶77．5），流速为1．0mL·min-1，检测波长为220nm（检测苦参碱）和250nm（检测甘草酸），进样量50μL。结果甘草酸单铵盐质量浓度在2．0～200.0μg·mL-1范围内线性良好，苦参碱质量浓度在2．0～200．0μg·mL-1范围内线性良好。平均回收率均大于95％，日内、日间RSD均小于4％。结论该方法简便、快速、灵敏、可靠，可用于人血浆中甘草酸和苦参碱质量浓度的监测。
목적：건립이HPLC법동시측정인혈장중감초산화고삼감질량농도적방법。방법Ultimate AQ-C18주（250mm×4.6mm，5μm），주온위실온（25℃），류동상위을정-삼을알조pH지7．5적0．05mol·L-1린산이경갑（22．5∶77．5），류속위1．0mL·min-1，검측파장위220nm（검측고삼감）화250nm（검측감초산），진양량50μL。결과감초산단안염질량농도재2．0～200.0μg·mL-1범위내선성량호，고삼감질량농도재2．0～200．0μg·mL-1범위내선성량호。평균회수솔균대우95％，일내、일간RSD균소우4％。결론해방법간편、쾌속、령민、가고，가용우인혈장중감초산화고삼감질량농도적감측。
Objective To establish an HPLC methods for the simultaneous determination of human plasma concentration of glycyr-rhizic acid and matrine .Methods Ultimate AQ-C18 (250 mm × 4 .6 mm ,5 μm) was chosen as the chromatographic column .The column temperature was at room temperature(25 ℃) .The mobile phase was composed of acetonitrile and 0 .05 mol · L -1 aqueous phosphoric acid(22 .5∶77 .5 ,adjusted to pH 7 .5 with triethylamine) .The flow rate was set at 1 .0 mL · min-1 .The detection wavelengths were set at 220 and 250 nm .The injection volume was 50μL .Results The linear ranges of glycyrrhizic acid ammoni-um salt and matrine were 2 .0-200 .0 μg · mL -1 and 2 .0-200 .0 μg · mL -1 ,respectively .The average recovery was above 95% ;both the intraday RSD and the interday RSD were less than 4% .Conclusions The method is simple ,rapid ,sensitive and reliable , and could be used for the simultaneous determination of human plasma concentration of glycyrrhizic acid and matrine .