CAJ | 학술논문

目的：建立维吾尔药材白花丹中白花丹醌含量测定方法。方法采用反相高效液相色谱法，色谱柱为Waters XTerra RP C18（250 mm ×4．6 mm ，5μm）；流动相为甲醇-水溶液；体积流量为1．0 mL · min-1；检测波长为270 nm ；柱温25℃。结果在上述条件下，白花丹醌进样量在0．0286～0．4576μg范围内呈良好的线性关系，加样回收率为100．4％，RSD为1.2％。结论该方法简便、准确、灵敏度高、重复性好，能有效测定白花丹药材中白花丹醌的含量。
목적：건립유오이약재백화단중백화단곤함량측정방법。방법채용반상고효액상색보법，색보주위Waters XTerra RP C18（250 mm ×4．6 mm ，5μm）；류동상위갑순-수용액；체적류량위1．0 mL · min-1；검측파장위270 nm ；주온25℃。결과재상술조건하，백화단곤진양량재0．0286～0．4576μg범위내정량호적선성관계，가양회수솔위100．4％，RSD위1.2％。결론해방법간편、준학、령민도고、중복성호，능유효측정백화단약재중백화단곤적함량。
Objective To establish a method for the determination of plumbagin in Plumbago zeylanica L ..Methods Plumbagin was determined by RP-HPLC on a Waters XTerra RP C18 (250 mm × 4 .6 mm ,5μm) column with methanol-water as the mobile phase . The flow rate was 1 .0 mL · min-1 ,the detection wavelength was 270 nm ,and the column temperature was 25 ℃ .Results The cal-ibration curve of plumbagin was linear within the range of 0 .028 6-0 .457 6 μg and the average recovery was 100 .4% (RSD=1.2% ) .Conclusion The method is simple ,accurate ,sensitive and reproducible .It could be used for the determination of plumba-gin in Plumbago zeylanica L ..