CAJ | 학술논문

牙骨质蛋白（CEMP）1是一种在牙骨质发生过程中的特异表达产物，是一种碱性蛋白质，其相对分子质量约2.59×104，重组人CEMP1的二级结构主要由β-折叠构成。将含CEMP1基因的载体转入人牙龈成纤维细胞（HGF），结果在HGF中检测出矿化细胞特有的钙化结节，同时也检出了羟磷灰石，其钙磷比值接近于生物型磷灰石。核心结合因子A1（CBFA1）是骨和软骨矿化所必需的转录因子，CEMP1可调节CBFA1的表达。经CEMP1处理的细胞呈组织样结构聚集，分层的细胞被细胞外基质包围，细胞稀疏地存在于小空腔中，这种形态特征跟软骨相似。具有高碱性磷酸酶（AKP）活性的人牙周膜细胞（hPDLC）有向成骨和成牙骨质细胞分化的能力，CEMP1仅在hPDLC和成牙骨质细胞中高表达，即CEMP1与hPDLC中高浓度的AKP有关，90%以上的成牙骨质细胞中有CEMP1强表达。本文就CEMP1，CEMP1的生物学作用，CEMP1的应用前景等研究进展作一综述。
아골질단백（CEMP）1시일충재아골질발생과정중적특이표체산물，시일충감성단백질，기상대분자질량약2.59×104，중조인CEMP1적이급결구주요유β-절첩구성。장함CEMP1기인적재체전입인아간성섬유세포（HGF），결과재HGF중검측출광화세포특유적개화결절，동시야검출료간린회석，기개린비치접근우생물형린회석。핵심결합인자A1（CBFA1）시골화연골광화소필수적전록인자，CEMP1가조절CBFA1적표체。경CEMP1처리적세포정조직양결구취집，분층적세포피세포외기질포위，세포희소지존재우소공강중，저충형태특정근연골상사。구유고감성린산매（AKP）활성적인아주막세포（hPDLC）유향성골화성아골질세포분화적능력，CEMP1부재hPDLC화성아골질세포중고표체，즉CEMP1여hPDLC중고농도적AKP유관，90%이상적성아골질세포중유CEMP1강표체。본문취CEMP1，CEMP1적생물학작용，CEMP1적응용전경등연구진전작일종술。
Cementum protein(CEMP)1, a specific product expressed during cementum formation, is an alkaline protein with a relative molecular mass of 2.59×104. The secondary structure present in recombinant human CEMP1(rhCEMP1) is mainly composed ofβ-sheet. Transfection of CEMP1 into human gingival fibroblasts promotes calcium nodule formation of hydroxyapatite that resembles the biological type, in Ca/P, which indicates cemetoblast and osteoblast phenotypes. CEMP1 can regulate core binding factorα1, a transcriptional activator of osteoblast differentiation. Co-culturing rhCEMP1 with human periodontal ligament cell (hPDLC) induces the formation of a tissue-like structure resembling bone and cementum, and cartilage-like material. Alkaline phosphatase(AKP)-positive hPDLC is preferentially categorized into cemetoblasts and osteoblasts. CEMP1 is strictly expressed in only a few hPDLC and >90% of cemetoblasts. Therefore, CEMP1 is closely related to the high AKP concentration in hPDLC. This article reviews the research progress in the biological function and future applications of CEMP1.