国际口腔医学杂志
國際口腔醫學雜誌
국제구강의학잡지
JOURNAL OF INTERNATIONAL STOMATOLOGY
2014年
2期
149-152
,共4页
刘莉%吕俊%谭颖徽%张纲
劉莉%呂俊%譚穎徽%張綱
류리%려준%담영휘%장강
高原%牙周炎%龈下菌斑%牙周致病菌
高原%牙週炎%齦下菌斑%牙週緻病菌
고원%아주염%간하균반%아주치병균
high altitude%periodontitis%subgingival plaque%periodontal pathogens
目的：分析高原地区与平原地区慢性牙周炎患者龈下菌斑中牙周致病菌的差异。方法应用以16S rRNA为基础的聚合酶链反应（PCR）技术，检测高原、平原地区的慢性牙周炎（CP）患者和牙周健康（PH）者龈下菌斑中6种牙周可疑致病菌，包括牙龈卟啉单胞菌（P. gingivalis）、福赛坦氏菌（T. forsythia）、齿垢密螺旋体（T. denticola）、伴放线放线杆菌（A. actinomycetemcomitans）、中间普氏菌（P. intermedia）和具核梭杆菌（F. nucleatum）。结果 高原CP组P. gingivalis、T. forsythia、T. denticola、A. actinomycetemcomitans、P. intermedia、F. nucleatum检出率分别为：90%、100%、80%、60%、85%、85%，明显高于PH组（P<0.05）；平原CP组P. gingivalis、T. forsythia、T. denticola、A. actinomycetemcomitans、P. intermedia、F. nucleatum检出率分别为80%、85%、70%、75%、50%，明显高于PH组（P<0.05）；F. nucleatum、A. actinomycetemcomitans在高原CP组的检出率高于平原CP组（P<0.05）。结论 6种细菌在高原CP患者龈下菌斑中均有较高检出率；高原地区与平原地区CP患者龈下菌斑中F. nucleatum、A. actinomycetemcomitans检出率有明显差异。
目的：分析高原地區與平原地區慢性牙週炎患者齦下菌斑中牙週緻病菌的差異。方法應用以16S rRNA為基礎的聚閤酶鏈反應（PCR）技術，檢測高原、平原地區的慢性牙週炎（CP）患者和牙週健康（PH）者齦下菌斑中6種牙週可疑緻病菌，包括牙齦卟啉單胞菌（P. gingivalis）、福賽坦氏菌（T. forsythia）、齒垢密螺鏇體（T. denticola）、伴放線放線桿菌（A. actinomycetemcomitans）、中間普氏菌（P. intermedia）和具覈梭桿菌（F. nucleatum）。結果 高原CP組P. gingivalis、T. forsythia、T. denticola、A. actinomycetemcomitans、P. intermedia、F. nucleatum檢齣率分彆為：90%、100%、80%、60%、85%、85%，明顯高于PH組（P<0.05）；平原CP組P. gingivalis、T. forsythia、T. denticola、A. actinomycetemcomitans、P. intermedia、F. nucleatum檢齣率分彆為80%、85%、70%、75%、50%，明顯高于PH組（P<0.05）；F. nucleatum、A. actinomycetemcomitans在高原CP組的檢齣率高于平原CP組（P<0.05）。結論 6種細菌在高原CP患者齦下菌斑中均有較高檢齣率；高原地區與平原地區CP患者齦下菌斑中F. nucleatum、A. actinomycetemcomitans檢齣率有明顯差異。
목적：분석고원지구여평원지구만성아주염환자간하균반중아주치병균적차이。방법응용이16S rRNA위기출적취합매련반응（PCR）기술，검측고원、평원지구적만성아주염（CP）환자화아주건강（PH）자간하균반중6충아주가의치병균，포괄아간계람단포균（P. gingivalis）、복새탄씨균（T. forsythia）、치구밀라선체（T. denticola）、반방선방선간균（A. actinomycetemcomitans）、중간보씨균（P. intermedia）화구핵사간균（F. nucleatum）。결과 고원CP조P. gingivalis、T. forsythia、T. denticola、A. actinomycetemcomitans、P. intermedia、F. nucleatum검출솔분별위：90%、100%、80%、60%、85%、85%，명현고우PH조（P<0.05）；평원CP조P. gingivalis、T. forsythia、T. denticola、A. actinomycetemcomitans、P. intermedia、F. nucleatum검출솔분별위80%、85%、70%、75%、50%，명현고우PH조（P<0.05）；F. nucleatum、A. actinomycetemcomitans재고원CP조적검출솔고우평원CP조（P<0.05）。결론 6충세균재고원CP환자간하균반중균유교고검출솔；고원지구여평원지구CP환자간하균반중F. nucleatum、A. actinomycetemcomitans검출솔유명현차이。
Objective This study was designed to investigate the differences among six periodontal pathogens from the subgingival plaque of chronic periodontitis(CP) patients living in high-altitude and plain areas. Methods Subgingival plaque samples were collected from 20 CP patients and 20 periodontal healthy(PH) subjects living in high-altitude or plain areas. Six periodontal pathogens, which includes Porphyromonas gingivalis(P. gingivalis), Tannerella forsythia(T. forsythia), Treponema denticola(T. denticola), Actinobacillus actinomycetemcomitans(A. actinomycetemcomitans), Prevotella intermedia(P. intermedia), and Fusobacterium nucleatum(F. nucleatum), were detected by 16S rRNA polymerase chain reaction. Results P. gingivalis, T. forsythia, T. denticola, A. actinomycetemcomitans, P. intermedia, and F. nucleatum were detected more frequently in high-altitude CP patients(with corresponding prevalence rates of 90%, 100%, 80%, 60% and 85%) compared with high-altitude PH controls(P<0.05). P. gingivalis, T. forsythia, T. denticola, P. intermedia, and F. nucleatum were detected more frequently in plain CP patients(with corresponding prevalence rates of 80%, 85%, 70%, 75% and 50%) than plain PH controls(P<0.05). The detection rates of F. nucleatum and A. actinomycetemcomitans in the high-altitude CP group were significantly higher than those in the plain CP group(P<0.05). Conclusion The high-altitude CP group showed the highest detection rates for the six periodontal pathogens than the other groups. Significant differences were found in the prevalence of F. nucleatum and A. actinomy-cetemcomitans between the high-altitude and plain CP groups.
