北方药学
北方藥學
북방약학
JOURNAL OF NORTH PHARMACY
2014年
3期
2-3
,共2页
三七丹胶囊%人参皂苷Rb1%人参皂苷Rg1%三七皂苷R1
三七丹膠囊%人參皂苷Rb1%人參皂苷Rg1%三七皂苷R1
삼칠단효낭%인삼조감Rb1%인삼조감Rg1%삼칠조감R1
Sanqidan Capsules%Ginsenoside Rb1%Ginsenoside Rg1%Notoginsenoside R1
目的:建立反相高效液相色谱法测定三七丹胶囊中人参皂苷Rb1、人参皂苷Rg1和三七皂苷R1的含量测定方法。方法:采用Hibar ODS C18(4.6mm×250mm,5μm)色谱柱,流动相为乙腈-水,梯度洗脱,流速1.0ml·min-1,柱温30℃,检测波长203nm。结果:人参皂苷Rb1、人参皂苷Rg1及三七皂苷R1分别在0.5246~5.2464μg(r=0.9997)、0.6792~6.7920μg(r=0.9998)和0.2542~2.5424μg(r=0.9997)范围内线性关系良好,平均回收率分别为人参皂苷Rb199.12%(RSD=0.61%,n=6)、人参皂苷Rg197.50%(RSD=1.63%,n=6)、三七皂苷R197.43%(RSD=1.04%,n=6)。结论:该方法定量简单、准确、重复性好,可作为三七丹胶囊的质量控制方法。
目的:建立反相高效液相色譜法測定三七丹膠囊中人參皂苷Rb1、人參皂苷Rg1和三七皂苷R1的含量測定方法。方法:採用Hibar ODS C18(4.6mm×250mm,5μm)色譜柱,流動相為乙腈-水,梯度洗脫,流速1.0ml·min-1,柱溫30℃,檢測波長203nm。結果:人參皂苷Rb1、人參皂苷Rg1及三七皂苷R1分彆在0.5246~5.2464μg(r=0.9997)、0.6792~6.7920μg(r=0.9998)和0.2542~2.5424μg(r=0.9997)範圍內線性關繫良好,平均迴收率分彆為人參皂苷Rb199.12%(RSD=0.61%,n=6)、人參皂苷Rg197.50%(RSD=1.63%,n=6)、三七皂苷R197.43%(RSD=1.04%,n=6)。結論:該方法定量簡單、準確、重複性好,可作為三七丹膠囊的質量控製方法。
목적:건립반상고효액상색보법측정삼칠단효낭중인삼조감Rb1、인삼조감Rg1화삼칠조감R1적함량측정방법。방법:채용Hibar ODS C18(4.6mm×250mm,5μm)색보주,류동상위을정-수,제도세탈,류속1.0ml·min-1,주온30℃,검측파장203nm。결과:인삼조감Rb1、인삼조감Rg1급삼칠조감R1분별재0.5246~5.2464μg(r=0.9997)、0.6792~6.7920μg(r=0.9998)화0.2542~2.5424μg(r=0.9997)범위내선성관계량호,평균회수솔분별위인삼조감Rb199.12%(RSD=0.61%,n=6)、인삼조감Rg197.50%(RSD=1.63%,n=6)、삼칠조감R197.43%(RSD=1.04%,n=6)。결론:해방법정량간단、준학、중복성호,가작위삼칠단효낭적질량공제방법。
Objective:To establish an RP-HPLC method for determination of Ginsenoside Rb1、Ginsenoside Rg1 and Notoginsenoside R1 in Sanqidan Capsules. Methods:Hibar ODS C18(4.6mm×250mm,5μm) column was used to determine Ginsenoside Rb1、Ginsenoside Rg1 and Notoginsenoside R1, with the mobile phase consisting of acetonitrile-water, gradient elution,the volume of flow 1.0 mL/min, column temperature 30℃ and detection wavelength 203nm. Restlts:The linear ranges of Ginsenoside Rb1、 Ginsenoside Rg1 and Notoginsenoside R1 were 0.5246~5.2464μg(r=0.9997)、0.6792~6.7920μg(r=0.9998) and 0.2542~2.5424μg(r=0.9997),respectively. The average recoveries of 99.12%(RSD=0.61%,n=6)for Ginsenoside Rb1,97.50%(RSD=1.63%,n=6) for Ginsenoside Rg1 and Notoginsenoside R1 97.43%(RSD=1.04%,n=6)was obtained. Conclusion:The method is simple,accurate and with good reproduccibility. It can be used for the quality control of Sanqidan Capsules.