CAJ | 학술논문

目的：研究骨肉瘤组织CD133、CD117及Ki-67在蛋白水平的表达情况及其与临床病理因素和危险度的关系。方法：应用免疫组织化学PV-9000二步法检测55例骨肉瘤组织标本中CD133、CD117和Ki-67的表达，并与临床病理学指标和术后无瘤生存期进行比较分析，对照组为20例骨软骨瘤。应用SPSS 17.0软件统计分析，检验标准为P<0.05具有统计学意义。结果：骨肉瘤组织CD133、CD117、Ki-67蛋白表达阳性率显著高于良性的骨软骨瘤组织，差异有统计学意义（分别为P=0.016、P=0.008、P<0.001）；CD133或Ki-67蛋白阳性表达骨肉瘤患者平均生存时间及平均转移时间短于CD133或Ki-67蛋白阴性骨肉瘤患者，差异有统计学意义（P<0.05）；CD133、Ki-67在外科分期和远处转移对骨肉瘤患者预后有影响，其中CD133是外科分期及远处转移而影响骨肉瘤患者预后的独立因素。结论：CD133和Ki-67表达可能在骨肉瘤发生、发展过程中起重要作用，有望成为判断其预后的指标。
목적：연구골육류조직CD133、CD117급Ki-67재단백수평적표체정황급기여림상병리인소화위험도적관계。방법：응용면역조직화학PV-9000이보법검측55례골육류조직표본중CD133、CD117화Ki-67적표체，병여림상병이학지표화술후무류생존기진행비교분석，대조조위20례골연골류。응용SPSS 17.0연건통계분석，검험표준위P<0.05구유통계학의의。결과：골육류조직CD133、CD117、Ki-67단백표체양성솔현저고우량성적골연골류조직，차이유통계학의의（분별위P=0.016、P=0.008、P<0.001）；CD133혹Ki-67단백양성표체골육류환자평균생존시간급평균전이시간단우CD133혹Ki-67단백음성골육류환자，차이유통계학의의（P<0.05）；CD133、Ki-67재외과분기화원처전이대골육류환자예후유영향，기중CD133시외과분기급원처전이이영향골육류환자예후적독립인소。결론：CD133화Ki-67표체가능재골육류발생、발전과정중기중요작용，유망성위판단기예후적지표。
Objective:This study aims to investigate the protein expression of CD133, CD117, and Ki-67 in human osteosarcoma tissues and explore their relationships with the clinico-pathological features and risk of osteosarcoma. Methods:Immunohistochemical method was used to examine the protein expression of CD133, CD117, and Ki-67 gene in the paraffin specimens of 55 and 20 cases of osteosarcoma and osteochondroma, respectively. SPSS17.0 statistical software was used to explore the relationships among the expressions of CD133, CD117, and Ki-67 gene and the biological behavior and prognosis of osteosarcoma. Test criterion:P<0.05 was considered statistically significant. Results:The positive expression rates of CD133, CD117, and Ki-67 were significantly higher in the osteosarcoma tissues than in the benign osteochondroma tissues, and the differences were significant (P=0.016, P=0.008, and P<0.001, respectively). The mean survival and metastasis time were shorter in the CD133 or Ki-67 positively expressed osteosarcoma patients than in those with CD133 or Ki-67 negatively expressed osteosarcoma. The differences were significant (P<0.05). The expression of CD133 and Ki-67, surgical staging, and distal metastasis were relevant to the prognosis of osteosarcoma patients. The expression of CD133 and distal metastasis are the independent risk factors that affect the prognosis of these patients. Conclusion:The positive expressions of CD133 and Ki-67 closely correlated with the occurrence and progression of osteosarcoma, and may be used as an indicator for prognosis of the cancer.