CAJ | 학술논문

背景：中药淫羊藿一直以来被用于骨质疏松的治疗和骨缺损的修复。目的：探讨淫羊藿对骨髓间充质干细胞成骨性分化的影响。 　　方法：应用数据库文献检索的方法获取淫羊藿对骨髓间充质干细胞成骨性分化影响研究的文献，对符合研究标准的文献进行深入的分析。通过检测淫羊藿诱导骨髓间充质干细胞成骨性分化过程中碱性磷酸酶、骨钙素、骨桥蛋白、转化生长因子β、骨形态发生蛋白、骨钙素、骨涎蛋白等，了解淫羊藿对骨髓间充质干细胞的增殖和成骨分化的机制。 　　结果与结论：淫羊藿对骨髓间充质干细胞的影响呈剂量依赖性，在诱导培养的早期淫羊藿苷可以提高细胞的磷酸酶活性，在诱导培养的晚期增加细胞钙化结节数，促进骨钙素分泌量，显著提高转化生长因子β1、骨形成蛋白2、胰岛样生长因子1、骨桥蛋白和骨涎蛋白等的表达水平。淫羊藿对骨髓间充质干细胞的增殖和成骨性分化有促进作用，可作为一种良好的骨诱导活性因子。
배경：중약음양곽일직이래피용우골질소송적치료화골결손적수복。목적：탐토음양곽대골수간충질간세포성골성분화적영향。 　　방법：응용수거고문헌검색적방법획취음양곽대골수간충질간세포성골성분화영향연구적문헌，대부합연구표준적문헌진행심입적분석。통과검측음양곽유도골수간충질간세포성골성분화과정중감성린산매、골개소、골교단백、전화생장인자β、골형태발생단백、골개소、골연단백등，료해음양곽대골수간충질간세포적증식화성골분화적궤제。 　　결과여결론：음양곽대골수간충질간세포적영향정제량의뢰성，재유도배양적조기음양곽감가이제고세포적린산매활성，재유도배양적만기증가세포개화결절수，촉진골개소분비량，현저제고전화생장인자β1、골형성단백2、이도양생장인자1、골교단백화골연단백등적표체수평。음양곽대골수간충질간세포적증식화성골성분화유촉진작용，가작위일충량호적골유도활성인자。
BACKGROUND:Epimedium has been used in the treatment of osteoporosis and repair of bone defects. OBJECTIVE:To explore the effects of epimedium on the osteogenic differentiation of bone marrow mesenchymal stem cells. METHODS:A database search was performed to retrieve literatures addressing epimedium effects on the osteogenic differentiation of bone marrow mesenchymal stem cells. Then, the papers meeting the criteria were selected for in-depth analysis. During the osteogenic differentiation induced by epimedium, alkaline phosphatase, osteocalcin, osteopontin, transforming growth factorβ, bone morphogenetic proteins, osteocalcin, bone sialoprotein were detected in the bone marrow mesenchymal stem cells to understand the underlying mechanism of epimedium in proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells. RESULTS AND CONCLUSION:Epimedium effects on bone marrow mesenchymal stem cells are shown in a dose-dependent manner. During the early induction, icari n can increase cellphosphatase activity;in the late induction, icari n can increase calcified nodules, promote osteocalcin secretion, significantly improve the expressions of transforming growth factorβ1, bone morphogenetic protein 2, insulin-like growth factor-1, osteopontin and bone sialoprotein. Epimedium, which can be used as an excellent osteoinductive factor, improves the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells.