CAJ | 학술논문

背景：目前研究表明，基质细胞衍生因子1在参与趋化迁移内源性神经干细胞中起着非常重要的作用，但其具体迁移机制尚不明确。 　　目的：观察外源性基质细胞衍生因子1对大鼠内源性神经干细胞的趋化迁移作用及海马区神经干细胞的激活增殖情况。 　　方法：通过向SD大鼠海马区上大脑皮质内注射外源性基质细胞衍生因子1(注射量为5μL，质量浓度为500μg/L)建立动物模型，于3，7，14，21 d后灌注取脑，通过石蜡切片免疫组织化学检测大鼠皮质内注射区及海马区nestin阳性细胞表达情况，并设实验对照组与空白对照组。 　　结果与结论：石蜡切片免疫组织化学显示：实验组注射区周围及海马区nestin表达阳性细胞的数量随时间推移逐渐增多，3，7 d时注射区及海马区nestin表达阳性细胞少量表达，14 d时注射区及海马区nestin表达阳性细胞进一步增多，并向注射区形成明显的迁移趋势，21 d时注射区及海马区nestin表达阳性细胞更多。实验对照组及空白实验组未见上述表现。结果表明外源性基质细胞衍生因子1可能诱导海马区神经干细胞的增殖分化，参与内源性神经干细胞的趋化迁移过程。
배경：목전연구표명，기질세포연생인자1재삼여추화천이내원성신경간세포중기착비상중요적작용，단기구체천이궤제상불명학。 　　목적：관찰외원성기질세포연생인자1대대서내원성신경간세포적추화천이작용급해마구신경간세포적격활증식정황。 　　방법：통과향SD대서해마구상대뇌피질내주사외원성기질세포연생인자1(주사량위5μL，질량농도위500μg/L)건립동물모형，우3，7，14，21 d후관주취뇌，통과석사절편면역조직화학검측대서피질내주사구급해마구nestin양성세포표체정황，병설실험대조조여공백대조조。 　　결과여결론：석사절편면역조직화학현시：실험조주사구주위급해마구nestin표체양성세포적수량수시간추이축점증다，3，7 d시주사구급해마구nestin표체양성세포소량표체，14 d시주사구급해마구nestin표체양성세포진일보증다，병향주사구형성명현적천이추세，21 d시주사구급해마구nestin표체양성세포경다。실험대조조급공백실험조미견상술표현。결과표명외원성기질세포연생인자1가능유도해마구신경간세포적증식분화，삼여내원성신경간세포적추화천이과정。
BACKGROUND:Stromal cellderived factor 1 in chemotactic migration of endogenous neural stem cells plays a very important role, but the specific migration mechanism is unclear OBJECTIVE:To observe the effects of exogenous stromal cellderived factor 1 on chemotactic migration and proliferation of neural stem cells in the rat hippocampus METHODS:Exogenous stromal cellderived factor 1 (5μL, 500μ/L) was injected into the hippocampus of Sprague-Dawley rats to establish animal models. Brain tissues were taken after days 3, 7, 14 and 21 of perfusion to prepare paraffin sections. Thereafter, nestin expression in the injection region and hippocampus was detected using immunohistochemical method. Experimental control and blank control groups were set. RESULTS AND CONCLUSION:Paraffin section immunohistochemical results displayed the number of nestin-positive cells in the injection and the hippocampus was gradual y increased. At 3 and 7 days, nestin expression was a little and increased at 14 days, forming a migration tendency to the injection region. At 21 days, there were more nestin-positive cells in the injection area and hippocampus. However, there were no changes as above in the experimental control and blank control groups. The results showed that exogenous stromal cellderived factor 1 may induce the proliferation of neural stem cells in the hippocampus and may be involved in chemotactic migration of endogenous neural stem cells.