CAJ | 학술논문

背景：研究发现人来源的骨髓细胞外基质能促进人牙周膜干细胞增殖并保持干细胞的特性。目的：初步观察人骨髓来源的细胞外基质对人牙周膜干细胞增殖能力影响。 　　方法：分离人正常牙周膜细胞及颌骨骨髓细胞，制备骨髓细胞外基质膜片。采用有限稀释法克隆培养纯化人牙周膜干细胞，透射电镜观察细胞超微结构。取P2代人牙周膜干细胞与骨髓细胞来源细胞外基质共同培养，以普通培养基对照，采用CCK8法及细胞周期流式技术检测人骨髓细胞来源细胞外基质对人牙周膜干细胞增殖能力的影响。 　　结果与结论：实验组人牙周膜干细胞较对照组相比细胞增殖能力显著增强(P<0.05)，且细胞符合其生物学形态及生物学特性，生长状态良好。说明实验建立起的骨髓细胞外基质能够促进牙周膜干细胞的增殖，是一种扩增干细胞的有效方法。
배경：연구발현인래원적골수세포외기질능촉진인아주막간세포증식병보지간세포적특성。목적：초보관찰인골수래원적세포외기질대인아주막간세포증식능력영향。 　　방법：분리인정상아주막세포급합골골수세포，제비골수세포외기질막편。채용유한희석법극륭배양순화인아주막간세포，투사전경관찰세포초미결구。취P2대인아주막간세포여골수세포래원세포외기질공동배양，이보통배양기대조，채용CCK8법급세포주기류식기술검측인골수세포래원세포외기질대인아주막간세포증식능력적영향。 　　결과여결론：실험조인아주막간세포교대조조상비세포증식능력현저증강(P<0.05)，차세포부합기생물학형태급생물학특성，생장상태량호。설명실험건립기적골수세포외기질능구촉진아주막간세포적증식，시일충확증간세포적유효방법。
BACKGROUND:Human bone marrow cells-derived extracellular matrix can promote proliferation of human periodontal ligament stem cells and maintain stem cellproperties. OBJECTIVE:To preliminarily investigate the effect of human bone marrow cells-derived extracellular matrix on the proliferation of human periodontal ligament stem cells. METHODS:Human periodontal ligament stem cells and bone marrow cells were separately derived from human periodontal tissue and jaw bone marrow, and human bone marrow cells-derived extracellular matrix was prepared. Human periodontal ligament stem cells were cultured and purified using limited dilution cloning method, and transmission electron microscope was used for ultrastructure observation. Human periodontal ligament stem cells at passage 2 were cultured with human bone marrow cells-derived extracellular matrix and normal culture medium (control group). The cellcounting kit-8 and flow cytometry were used to determine the proliferation potential of human periodontal ligament stem cells cultured on human bone marrow cells-derived extracellular matrix. RESULTS AND CONCLUSION:Compared with the control group, human periodontal ligament stem cells cultured on human bone marrow cells-derived extracellular matrix had a superior capacity of proliferation (P<0.05), and the cells met their morphological and biological characteristics, and grew in good conditions. Human bone marrow cells-derived extracellular matrix is a promising matrix for large-scale expanding human periodontal ligament stem cells for future use in stem cel-based therapy.