CAJ | 학술논문

背景：目前骨髓动员能否归巢到心肌病心衰受损部位，分化成心肌样细胞和血管内皮细胞尚无定论。目的：观察骨髓动员剂粒细胞集落刺激因子对心肌病心衰大鼠心肌和血管的影响。 　　方法：选取阿霉素心肌病心衰模型大鼠50只，分成2组，骨髓动员组30只皮下注射重组人粒细胞集落刺激因子，心衰组20只给予相同体积的生理盐水。骨髓动员组其中10只在动员第6天处死，留取心肌标本，行CD34免疫荧光检测。各组在治疗前及治疗后第5天剪尾取血，测量白细胞总数及单个核细胞百分比，同时提取外周血中单个核细胞行流式细胞仪检测。治疗第5天，腹腔注射Brdu 50 mg/kg，连续4周直至动物处死。留取心肌组织，通过心肌BrdU单染、BrdU与肌球蛋白重链双染及BrdU与肌动蛋白双染确定单个核细胞的归巢，评价移植的单个核细胞向心肌和血管内皮细胞的生成、分化。采用苏木精-伊红染色评价血管密度。 　　结果与结论：骨髓动员组白细胞数及单个核细胞数较治疗前明显增高(P<0.05)。流式细胞仪证实骨髓动员组外周血单个核细胞CD34阳性率显著高于心衰组(P<0.05)。心肌CD34免疫荧光检测，心衰组阴性，骨髓动员组阳性。骨髓动员组心肌BrdU单染和肌球蛋白重链、肌动蛋白双染均呈阳性，心肌损伤处血管内皮细胞核呈BrdU阳性；心衰组均为阴性。骨髓动员组血管密度显著高于心衰组(P<0.001)。提示骨髓动员出单个核细胞，归巢到心肌受损处，对心肌病心衰大鼠模型心肌和血管有明显修复作用。
배경：목전골수동원능부귀소도심기병심쇠수손부위，분화성심기양세포화혈관내피세포상무정론。목적：관찰골수동원제립세포집락자격인자대심기병심쇠대서심기화혈관적영향。 　　방법：선취아매소심기병심쇠모형대서50지，분성2조，골수동원조30지피하주사중조인립세포집락자격인자，심쇠조20지급여상동체적적생리염수。골수동원조기중10지재동원제6천처사，류취심기표본，행CD34면역형광검측。각조재치료전급치료후제5천전미취혈，측량백세포총수급단개핵세포백분비，동시제취외주혈중단개핵세포행류식세포의검측。치료제5천，복강주사Brdu 50 mg/kg，련속4주직지동물처사。류취심기조직，통과심기BrdU단염、BrdU여기구단백중련쌍염급BrdU여기동단백쌍염학정단개핵세포적귀소，평개이식적단개핵세포향심기화혈관내피세포적생성、분화。채용소목정-이홍염색평개혈관밀도。 　　결과여결론：골수동원조백세포수급단개핵세포수교치료전명현증고(P<0.05)。류식세포의증실골수동원조외주혈단개핵세포CD34양성솔현저고우심쇠조(P<0.05)。심기CD34면역형광검측，심쇠조음성，골수동원조양성。골수동원조심기BrdU단염화기구단백중련、기동단백쌍염균정양성，심기손상처혈관내피세포핵정BrdU양성；심쇠조균위음성。골수동원조혈관밀도현저고우심쇠조(P<0.001)。제시골수동원출단개핵세포，귀소도심기수손처，대심기병심쇠대서모형심기화혈관유명현수복작용。
BACKGROUND:It is controversial whether bone marrow mobilization can retain in cardiac injured position in congestive cardiomyopathy or differentiate into cardiomyocytes and vascular endothelial cells. OBJECTIVE:To study the effects of granulocyte colony stimulating factor (G-GSF) on myocardium and angiogenesis in rats with congestive cardiomyopathy. METHODS:Fifty Wistar rats with heart failure caused by adriamycin-induced cardiomyopathy were divided into heart failure group (n=20) treated with normal saline and bone marrow mobilization (n=30) treated with subcutaneous injection of recombinant human G-GSF. Ten rats from the bone marrow mobilization were kil ed at day 6 of mobilization, and myocardial tissue was taken for CD43 immunofluorescent staining. Blood samples were taken from the rat tail in each group before and 5 days after treatment to count total number of white blood cells and percentage of mononuclear cells. Meanwhile, mononuclear cells extracted from the peripheral blood were used for flow cytometry detection. At day 5 after treatment, bromodeoxyuridine (BrdU, 50 mg/kg) was successively given to al rats for 4 weeks before they were sacrificed. Myocardial tissues were taken to determine the homing of mononuclear cells and evaluate differentiation of mononuclear cells into cardiomyocytes and vascular endothelial cells using BrdU staining, BrdU/myosin heavy chain double staining, and BrdU/actin double staining. Hematoxylin-eosin staining was used for determination of blood vessel density. RESULTS AND CONCLUSION:G-CSF mobilization increased the number of mononuclear cells that was significantly higher than before treatment (P<0.05). Flow cytometry showed that the number of CD34-positive mononuclear cells in the peripheral blood was higher in the bone marrow mobilization than in the heart failure group (P<0.05). Myocardial CD34 immunofluorescence showed that the heart failure group was negative and the bone marrow mobilization group was positive. In the bone marrow mobilization group, the myocardial tissue was positive for BrdU staining, BrdU/myosin heavy chain double staining and BrdU/actin double staining, while vascular endothelial cells in the region of myocardial injury was positive for BrdU;conversely, the heart failure group was negative. The density of blood vessels in the bone marrow mobilization group was significantly higher than that in the heart failure group (P<0.001). These findings indicate that bone marrow mobilization increases the number of mononuclear cells, and these cells are homing to myocardial injury, thereby playing a repair role in the myocardium and vascular tissue of heart failure rats with congestive cardiomyopathy.