CAJ | 학술논문

背景：骨髓间充质干细胞移植可以通过旁分泌机制改变周围区微环境，通过促进血管新生、抑制细胞凋亡和维护自主神经功能稳定等作用，可用于治疗肺功能严重损害。 　　目的：通过骨髓间充质干细胞移植，观察其对实验性肺气肿炎症反应及细胞凋亡的抑制作用。 　　方法：24只Wistar雌性大鼠随机分健康对照组、模型组及实验组，后两组烟熏加气管内滴注猪胰弹性蛋白酶法建立肺气肿模型。建模后大鼠尾静脉注射骨髓间充质干细胞，观察细胞移植后大鼠肺组织的病理学变化、检测支气管肺泡灌洗液中细胞数及肿瘤坏死因子水平、肺泡壁细胞凋亡指数。 　　结果与结论：模型组、实验组大鼠的肺组织都存在不同程度的肺气肿病理改变，实验组较轻，差异有显著性意义(P<0.01)；实验组肿瘤坏死因子水平及肺组织细胞凋亡指数均低于模型组，差异有显著性意义(P<0.01)。提示骨髓间充质干细胞通过抑制实验性肺气肿炎症反应及细胞凋亡，改善了肺气肿病理学，对肺气肿有明显的治疗作用。
배경：골수간충질간세포이식가이통과방분비궤제개변주위구미배경，통과촉진혈관신생、억제세포조망화유호자주신경공능은정등작용，가용우치료폐공능엄중손해。 　　목적：통과골수간충질간세포이식，관찰기대실험성폐기종염증반응급세포조망적억제작용。 　　방법：24지Wistar자성대서수궤분건강대조조、모형조급실험조，후량조연훈가기관내적주저이탄성단백매법건립폐기종모형。건모후대서미정맥주사골수간충질간세포，관찰세포이식후대서폐조직적병이학변화、검측지기관폐포관세액중세포수급종류배사인자수평、폐포벽세포조망지수。 　　결과여결론：모형조、실험조대서적폐조직도존재불동정도적폐기종병리개변，실험조교경，차이유현저성의의(P<0.01)；실험조종류배사인자수평급폐조직세포조망지수균저우모형조，차이유현저성의의(P<0.01)。제시골수간충질간세포통과억제실험성폐기종염증반응급세포조망，개선료폐기종병이학，대폐기종유명현적치료작용。
BACKGROUND:Bone marrow mesenchymal stem cells transplantation can change the surrounding microenvironment through paracrine mechanisms, and can be employed for treatment of serious damage to lung function through the promotion of angiogenesis, inhibition of apoptosis and maintaining functional stability of autonomic nervous system. OBJECTIVE:To observe the inflammatory reaction in experimental emphysema and inhibition of apoptosis through bone marrow mesenchymal stem cells transplantation. METHODS:Twenty-four Wistar female rats were randomly divided into three groups:healthy control group, model group and experimental group. In the latter two groups, smoking and endotracheal instil ation of porcine pancreatic elastase were performed to establish emphysema models. After modeling, bone marrow mesenchymal stem cells were injected via tail vein in the experimental group. Pathological changes of the lung, the level of tumor necrosis factor-alpha and cellnumber in the bronchoalveolar lavage fluid as wel as apoptotic index in lveolar wal s were detected after celltransplantation. RESULTS AND CONCLUSION:In the model and experimental groups, pathological changes of lung tissues were observed to different extent. The lung pathological changes were slighter in the experimental group than the model group (P<0.01). The level of tumor necrosis factor-alpha and apoptotic index in lung tissue were lower in the experimental group than the model group (P<0.01). These findings indicate that bone marrow mesenchymal stem cells can improve emphysema pathological y through inhibition of inflammatory response and apoptosis in experimental emphysema.