CAJ | 학술논문

背景：前期初步研究发现，碱性成纤维细胞生长因子可诱导骨髓间充质干细胞向颞下颌关节盘细胞方向分化，且碱性成纤维细胞生长因子10μg/L诱导组合成胶原量明显高于5μg/L诱导组。 　　目的：观察经不同浓度碱性成纤维细胞生长因子诱导后的骨髓间充质干细胞的超微结构变化。 　　方法：原代分离培养山羊骨髓间充质干细胞，选P3，P4细胞，用5，10μg/L碱性成纤维细胞生长因子诱导骨髓间充质干细胞，以未加碱性成纤维细胞生长因子培养的骨髓间充质干细胞做对照。倒置相差显微镜观察细胞生长状况，用第7，14，21天的细胞爬片行蕃红O、天狼猩红和Ⅰ型胶原免疫组织化学染色，并观察第21天细胞的超微结构。 　　结果与结论：经不同浓度碱性成纤维细胞生长因子诱导后，骨髓间充质干细胞可向颞下颌关节盘成纤维细胞样细胞形态分化，10μg/L组细胞更像关节盘成纤维细胞样细胞。提示骨髓间充质干细胞有向颞下颌关节盘细胞方向分化的形态学基础。
배경：전기초보연구발현，감성성섬유세포생장인자가유도골수간충질간세포향섭하합관절반세포방향분화，차감성성섬유세포생장인자10μg/L유도조합성효원량명현고우5μg/L유도조。 　　목적：관찰경불동농도감성성섬유세포생장인자유도후적골수간충질간세포적초미결구변화。 　　방법：원대분리배양산양골수간충질간세포，선P3，P4세포，용5，10μg/L감성성섬유세포생장인자유도골수간충질간세포，이미가감성성섬유세포생장인자배양적골수간충질간세포주대조。도치상차현미경관찰세포생장상황，용제7，14，21천적세포파편행번홍O、천랑성홍화Ⅰ형효원면역조직화학염색，병관찰제21천세포적초미결구。 　　결과여결론：경불동농도감성성섬유세포생장인자유도후，골수간충질간세포가향섭하합관절반성섬유세포양세포형태분화，10μg/L조세포경상관절반성섬유세포양세포。제시골수간충질간세포유향섭하합관절반세포방향분화적형태학기출。
BACKGROUND:Our preliminary studies have shown that basic fibroblast growth factor can induce the differentiation of bone marrow mesenchymal stem cells into disc cells of the temporomandibular joint, and for basic fibroblast growth factor, 10μg/L is superior to 5μg/L in col agen synthesis. OBJECTIVE:To observe ultrastructural changes of bone marrow mesenchymal stem cells after being induced by different concentrations of basic fibroblast growth factor. METHODS:We cultured primary sheep bone marrow mesenchymal stem cells and selected passage 3 and 4 cells at good growth state. Bone marrow mesenchymal stem cells were stimulated with 5 and 10μg/L basic fibroblast growth factor and their growth state was observed under inverted phase contrast microscope. Uninduced cells served as controls. The slides with cellcrawling pieces were stained with Safranin O, picrosirius and type I col agen immunohistochemistry at days 7, 14 and 21, respectively. Simultaneously we col ected the cells at day 21 to observe the ultrastructural changes of bone marrow mesenchymal stem cells. RESULTS AND CONCLUSION:After being induced with different concentrations of basic fibroblast growth factor, bone marrow mesenchymal stem cells were able to differentiate into disc cells of the temporomandibular joint;and after being induced with 10μg/L basic fibroblast growth factor, cells were more like fibroblast-like cells of the temporomandibular joint disc. These findings indicate that bone marrow mesenchymal stem cells have morphological basis for differentiation to the fibroblast-like cells of the temporomandibular joint disc.