CAJ | 학술논문

背景：尿酸作为一种内源性的抗氧化剂，其抗氧化、抗 DNA 损害作用及发挥神经元保护作用近年受到关注。 　　目的：观察不同浓度尿酸对骨髓间充质干细胞成神经分化的影响。 　　方法：体外分离、纯化、培养骨髓间充质干细胞，观察细胞形态，取扩增3代的骨髓间充质干细胞，分别用含0(对照组)、0.2，0.4，0.8 mmol/L浓度尿酸的预诱导液诱导24 h，再用含0(对照组)、0.2，0.4，0.8 mmol/L浓度尿酸的诱导液诱导1 h后行尼氏体染色，2 h后，用免疫组织化学法检测细胞内巢蛋白、神经元特异性烯醇化酶的表达。 　　结果与结论：骨髓间充质干细胞经诱导后，细胞胞体收缩，形成突起并形成连接。细胞胞质中存在蓝色颗粒状的尼氏体，含不同浓度尿酸的诱导组神经元特异性烯醇化酶阳性率均较对照组明显升高(P <0.05)，而且随着尿酸浓度增加，神经元特异性烯醇化酶阳性表达率逐渐增加(P <0.05)。含不同浓度尿酸的诱导组巢蛋白阳性表达率较对照组降低(P<0.05)，诱导4 h后，细胞脱落明显。在体外一定时间内，尿酸能够促进骨髓间充质干细胞向神经元样细胞的分化，且具有一定的浓度依赖性。
배경：뇨산작위일충내원성적항양화제，기항양화、항 DNA 손해작용급발휘신경원보호작용근년수도관주。 　　목적：관찰불동농도뇨산대골수간충질간세포성신경분화적영향。 　　방법：체외분리、순화、배양골수간충질간세포，관찰세포형태，취확증3대적골수간충질간세포，분별용함0(대조조)、0.2，0.4，0.8 mmol/L농도뇨산적예유도액유도24 h，재용함0(대조조)、0.2，0.4，0.8 mmol/L농도뇨산적유도액유도1 h후행니씨체염색，2 h후，용면역조직화학법검측세포내소단백、신경원특이성희순화매적표체。 　　결과여결론：골수간충질간세포경유도후，세포포체수축，형성돌기병형성련접。세포포질중존재람색과립상적니씨체，함불동농도뇨산적유도조신경원특이성희순화매양성솔균교대조조명현승고(P <0.05)，이차수착뇨산농도증가，신경원특이성희순화매양성표체솔축점증가(P <0.05)。함불동농도뇨산적유도조소단백양성표체솔교대조조강저(P<0.05)，유도4 h후，세포탈락명현。재체외일정시간내，뇨산능구촉진골수간충질간세포향신경원양세포적분화，차구유일정적농도의뢰성。
BACKGROUND:Uric acid as an endogenous antioxidant has garnered increasing attentions because of its anti-oxidation, anti-DNA damage and neuroprotective effects. OBJECTIVE:To observe the effect of uric acid at different concentrations on the neural differentiation of bone marrow mesenchymal stem cells. METHODS:Bone marrow mesenchymal stem cells were isolated, purified and cultured in vitro. The morphology change was observed. The third passages of bone marrow mesenchymal stem cells were induced to differentiate to neuron-like cells by induced liquid containing four different concentrations of uric acid (0 mmol/L as control group, 0.2 mmol/L, 0.4 mmol/L, 0.8 mmol/L) for 24 hours. Then, after second intervention for 1 hour, cells were detected by Nissl staining and specific markers were detected by immunohistochemistry method. RESULTS AND CONCLUSION:After induction, the cellbody shrank, forming processes and connections. Nissl body was found in the cytoplasm. The positive rates of neuron-specific enolase were significantly higher in uric acid groups of different concentrations compared to the control group (P<0.05);moreover, the positive rates of neuron-specific enolase were increased as the increase in concentrations of uric acid (P<0.05). The positive rates of Nestin were decreased in uric acid groups of different concentrations compared to the control group (P<0.05). After 4 hours of induction, cells fel off significantly. In a certain period of time, uric acid can promote differentiation of bone marrow mesenchymal stem cells into neuron-like cells in a certain concentration-dependent manner in vitro.