CAJ | 학술논문

背景：研究证实尼古丁会影响成骨细胞、破骨细胞、成纤维细胞和红细胞的活性。 　　目的：检测尼古丁对表面喷砂或酸蚀处理种植体植入后骨结合及骨保护素、骨形成蛋白2表达的影响。 　　方法：将24只SD大鼠随机均分为实验组和对照组，实验组大鼠每天2次背部皮下注射2 mg/kg尼古丁，对照组对应皮下注射等量生理盐水。2周后在两组大鼠胫骨近干骺端分别植入表面喷砂或酸蚀处理的钛种植体，实验组继续皮下注射尼古丁，对照组注射生理盐水。种植后第2，4周对种植体及其周围骨组织行CT、X射线、荧光定量PCR及苏木精-伊红染色观察。 　　结果与结论：与对照组相比，实验组骨结合程度及骨保护素和骨形成蛋白2表达明显下降(P <0.05)。在尼古丁作用下，表面喷砂处理种植体组骨保护素和骨形成蛋白2表达、表面酸蚀处理种植体组骨保护素表达均随时间变化明显下调(P <0.05)，并且表面酸蚀处理种植体组植入2周时骨形成蛋白2表达高于表面喷砂处理种植体组(P <0.05)；X射线与CT结果提示，尼古丁干预对表面酸蚀处理种植体周围新生骨形成量和新生骨矿化程度的影响明显小于表面喷砂处理种植体。苏木精-伊红染色显示，两种种植体周围成骨细胞的数量与活性随时间变化均降低，但表面酸蚀处理种植体组效果好于表面喷砂处理种植体组。
배경：연구증실니고정회영향성골세포、파골세포、성섬유세포화홍세포적활성。 　　목적：검측니고정대표면분사혹산식처리충식체식입후골결합급골보호소、골형성단백2표체적영향。 　　방법：장24지SD대서수궤균분위실험조화대조조，실험조대서매천2차배부피하주사2 mg/kg니고정，대조조대응피하주사등량생리염수。2주후재량조대서경골근간후단분별식입표면분사혹산식처리적태충식체，실험조계속피하주사니고정，대조조주사생리염수。충식후제2，4주대충식체급기주위골조직행CT、X사선、형광정량PCR급소목정-이홍염색관찰。 　　결과여결론：여대조조상비，실험조골결합정도급골보호소화골형성단백2표체명현하강(P <0.05)。재니고정작용하，표면분사처리충식체조골보호소화골형성단백2표체、표면산식처리충식체조골보호소표체균수시간변화명현하조(P <0.05)，병차표면산식처리충식체조식입2주시골형성단백2표체고우표면분사처리충식체조(P <0.05)；X사선여CT결과제시，니고정간예대표면산식처리충식체주위신생골형성량화신생골광화정도적영향명현소우표면분사처리충식체。소목정-이홍염색현시，량충충식체주위성골세포적수량여활성수시간변화균강저，단표면산식처리충식체조효과호우표면분사처리충식체조。
BACKGROUND:Studies have confirmed that nicotine affects the activity of osteoblasts, osteoclasts, fibroblasts and erythrocytes. OBJECTIVE:To study the nicotine effects on osseointegration and the expression of osteoprotegerin and bone morphogenetic protein 2 after implantation of dental implants with surface treatment by sandblasting or acid etching. METHODS:Twenty-four Sprague-Dawley rats were randomly assigned to two groups and received daily injections for 2 weeks as folows: Nicotine 2 mg/kg twice for experimental group, saline solution for control group. Then the titanium implants with surface sandblasting or acid etching were implanted into the tibiae folowed by continuous nicotine or normal saline injection. At weeks 2 and 4 after implantation, the implants and surrounding bone tissue were prepared for CT, X-ray and hematoxylin-eosin staining examinations to evaluate bone healing and expression levels of bone-related genes were measured by quantitative RT-PCR. RESULTS AND CONCLUSION: Compared with the control groups, the degree of osseointegration and the expression of osteoprotegerin and bone morphogenetic protein 2 in the experimental groups were decreased significantly (P < 0.05), except that the expression of bone morphogenetic protein 2 in the experimental group with acid etching was not significantly reduced. In addition, the expression of bone morphogenetic protein 2 in the experimental group with acid etching was higher than that in the experimental group with sandblasting at 2 weeks after implantation (P < 0.05). The X-ray and CT show that the quantities of new generation bone and the degree of bone mineralization of the sandblasting group were significant lower than those of the acid etching group under the intervention of nicotine. Hematoxylin-eosin staining showed that the activity and quantity of osteoblasts around the implants down-regulated significantly, but acid etching-treated implants showed better outcomes than sandblasting-treated implants.