CAJ | 학술논문

以海南陵水、马来西亚、西沙、南沙4个海域共101尾波纹唇鱼（Cheilinus undulatus）作为研究对象，利用线粒体DNA ND1基因序列对波纹唇鱼进行了遗传多样性分析。通过PCR扩增、克隆与序列测定技术，获得ND1基因序列长度为975 bp，其多态性遗传参数统计显示，101个个体存在13个变异位点，14个单倍型，总群体单倍型多样性（Hd）为0.292，平均核苷酸差异数（K）为0.335，核苷酸多样性指数（Pi）为0.00035，表现出遗传多样性处于较低水平。Tajima's D中性检验结果为-1.76809（P=0.031）表明波纹唇鱼在历史上经历过近期群体扩张或瓶颈效应。分子方差分析（AMOVA）表明遗传变异来自群体内。该结果可为今后波纹唇鱼的种质资源保护工作提供必要的科学依据。
이해남릉수、마래서아、서사、남사4개해역공101미파문진어（Cheilinus undulatus）작위연구대상，이용선립체DNA ND1기인서렬대파문진어진행료유전다양성분석。통과PCR확증、극륭여서렬측정기술，획득ND1기인서렬장도위975 bp，기다태성유전삼수통계현시，101개개체존재13개변이위점，14개단배형，총군체단배형다양성（Hd）위0.292，평균핵감산차이수（K）위0.335，핵감산다양성지수（Pi）위0.00035，표현출유전다양성처우교저수평。Tajima's D중성검험결과위-1.76809（P=0.031）표명파문진어재역사상경력과근기군체확장혹병경효응。분자방차분석（AMOVA）표명유전변이래자군체내。해결과가위금후파문진어적충질자원보호공작제공필요적과학의거。
In this study, the genetic diversity and divergence of Cheilinus undulatus were studied based on the analysis of mtDNA ND1 gene sequence from 101 individuals of four geographical populations including those from Lingshui (n=31), Malaysia (n=20), Xisha (n=14), and Nansha (n=36). By the PCR and cloning techniques, we got 975 bp sequences of the ND1 gene. Thirteen variable sites and fourteen haplotypes were obtained in this study. The whole haplotype diversity (Hd) of all individuals was 0.292, and the average nucleotide differences (K) and nucleotide diversities (Pi) were 0.335 and 0.000 35, respectively. The results demonstrated that the genetic diversity was low. The data of Tajimas'D test (-1.768 09, P=0.031) suggested that Cheilinus undulatus underwent through recent group expansion or bottleneck effect. The AMOVA analysis showed that a large proportion of variation was contributed within the population of Cheilinus undulatus. These results might be very useful for the protective measures for this species.