CAJ | 학술논문

[目的]探讨缺氧诱导因子-1α（hypoxia inducible factor-1α，HIF-1α）对间充质干细胞基质细胞衍生因子-1（stromal cel-derived factor-1， SDF-1）/ CXC趋化因子受体4（chemokine receptor 4，CXCR4）的调控作用。[方法]贴壁法培养ICR小鼠骨髓间充质干细胞（mesenchymal stem cel s，MSCs）；采用RNA干扰技术用SuperFectinTMⅡ转染HIF-1α siRNA于MSCs；实验分为五组：常氧组、缺氧组、转染Control siRNA组、转染HIF-1αsiRNA常氧培养组、转染HIF-1αsiRNA缺氧培养组；RT-PCR检测HIF-1α、SDF-1α、CXCR4 mRNA表达水平；Western blotting检测HIF-1α、SDF-1α、CXCR4蛋白表达水平。[结果]同常氧组比较，缺氧组HIF-1α、SDF-1α、CXCR4 mRNA及蛋白表达水平提高（P<0.05）；同转染Control siRNA组比较，转染HIF-1α siRNA常氧培养组HIF-1α、SDF-1α、CXCR4 mRNA及蛋白表达水平降低（P<0.05）；同缺氧组比较，转染HIF-1αsiRNA缺氧培养组HIF-1α、SDF-1α、CXCR4 mRNA及蛋白表达水平降低（P<0.05）。[结论]缺氧可使MSCs的HIF-1α、SDF-1α、CX-CR4的表达提高，常氧与缺氧条件下抑制HIF-1α的表达可使SDF-1α、CXCR4的表达降低，HIF-1α在间充质干细胞中对SDF-1/CXCR4有调控作用。
[목적]탐토결양유도인자-1α（hypoxia inducible factor-1α，HIF-1α）대간충질간세포기질세포연생인자-1（stromal cel-derived factor-1， SDF-1）/ CXC추화인자수체4（chemokine receptor 4，CXCR4）적조공작용。[방법]첩벽법배양ICR소서골수간충질간세포（mesenchymal stem cel s，MSCs）；채용RNA간우기술용SuperFectinTMⅡ전염HIF-1α siRNA우MSCs；실험분위오조：상양조、결양조、전염Control siRNA조、전염HIF-1αsiRNA상양배양조、전염HIF-1αsiRNA결양배양조；RT-PCR검측HIF-1α、SDF-1α、CXCR4 mRNA표체수평；Western blotting검측HIF-1α、SDF-1α、CXCR4단백표체수평。[결과]동상양조비교，결양조HIF-1α、SDF-1α、CXCR4 mRNA급단백표체수평제고（P<0.05）；동전염Control siRNA조비교，전염HIF-1α siRNA상양배양조HIF-1α、SDF-1α、CXCR4 mRNA급단백표체수평강저（P<0.05）；동결양조비교，전염HIF-1αsiRNA결양배양조HIF-1α、SDF-1α、CXCR4 mRNA급단백표체수평강저（P<0.05）。[결론]결양가사MSCs적HIF-1α、SDF-1α、CX-CR4적표체제고，상양여결양조건하억제HIF-1α적표체가사SDF-1α、CXCR4적표체강저，HIF-1α재간충질간세포중대SDF-1/CXCR4유조공작용。
[Aim] To study the regulation of HIF-1α for SDF-1/CXCR4 in MSCs. [Methods] MSCs were cultured by adherence method. HIF-1αsiRNA was transfected to MSCs by SuperFectinTM Ⅱ. The experiment was divided into five groups, normoxia group,hypoxia group,control siRNA transfected group, HIF-1α siRNA transfected normoxic group, HIF-1α siRNA transfected hypoxia group. Reverse transcdption-polymerase chain reaction was utilized to determine HIF-1α, SDF-1α, CXCR4 mRNA expression in MSCs.Western blotting was employed to detect HIF-1α, SDF-1α, CXCR4 protein expression in MSCs. [Result] Compared with the normoxia group, HIF-1α, SDF-1α and CXCR4 mRNA and protein expression increased in the hypoxia group(P<0.05). Compared with the control siRNA transfected group, HIF-1α, SDF-1α and CXCR4 mRNA and protein expression decreased in the HIF-1α siRNA transfected normoxic group(P<0.05); Compared with the hypoxia group, HIF-1α, SDF-1α and CXCR4 mRNA and protein expression decreased in the HIF-1α siRNA transfected hypoxia group(P<0.05). [Conclusion] These results verified that the expressions of HIF-1α, SDF-1αand CXCR4 were increased in hypoxic environment. Inhibition of HIF-1αexpression decreased SDF-1αand CXCR4 expression both in normoxia and hypoxia. HIF-1αplays an important role in regulating SDF-1/CXCR4 in mesenchymal stem cells.