CAJ | 학술논문

为探索滇杨花粉长期保存的适宜贮藏条件，采用离体培养法筛选滇杨花粉萌发适宜的蔗糖浓度，在适宜蔗糖浓度基础上，研究不同贮藏温度和时间滇杨花粉萌发率的变化。结果表明：滇杨花粉在1％琼脂＋15 g／L 蔗糖＋0．01％硼酸的培养基上萌发率最高，达34．84％；花粉萌发率因贮藏温度不同存在显著差异，在-80℃下贮藏240 d，萌发率为28．35％，显著高于同时期内其他温度贮藏处理；花粉萌发率随贮藏时间的延长呈下降趋势，相同时间内贮藏温度越低花粉萌发率越高，室温下花粉萌发率下降最快。
위탐색전양화분장기보존적괄의저장조건，채용리체배양법사선전양화분맹발괄의적자당농도，재괄의자당농도기출상，연구불동저장온도화시간전양화분맹발솔적변화。결과표명：전양화분재1％경지＋15 g／L 자당＋0．01％붕산적배양기상맹발솔최고，체34．84％；화분맹발솔인저장온도불동존재현저차이，재-80℃하저장240 d，맹발솔위28．35％，현저고우동시기내기타온도저장처리；화분맹발솔수저장시간적연장정하강추세，상동시간내저장온도월저화분맹발솔월고，실온하화분맹발솔하강최쾌。
Effects of different sucrose concentrations on pollen germination rates of P .yunnanensis in vitroculture were investigated.According to the result,germination rates of pollen stored under different temperature and time were tested. It aimed to explore the suitable storage method for long-term preservation.The results showed that the highest pollen germination rates of P .yunnanensis was 34.84%on the medium composed of 1% ager,15 g/L sucrose and 0.01% boric acid.Significant differences were observed among pollen stored under different temperatures.After 240 days of storage at 80℃ pollen germination rate was 28.35%,which was significantly higher than pollen germination rates stored under the other three temperatures.It also showed that with the extension of storage time,pollen viability decreased,and at the same time the storage temperature lower,pollen viability was higher,the fastest decline in pollen viability at room temperature.