贵州农业科学
貴州農業科學
귀주농업과학
GUIZHOU AGRICULTURAL SCIENCES
2014年
6期
76-78,79
,共4页
徐胜男%王济%张凌云%张施施%李珊珊%邓赞
徐勝男%王濟%張凌雲%張施施%李珊珊%鄧讚
서성남%왕제%장릉운%장시시%리산산%산찬
马缨杜鹃%菌根真菌%分子生物学%rDNA-ITS-PCR
馬纓杜鵑%菌根真菌%分子生物學%rDNA-ITS-PCR
마영두견%균근진균%분자생물학%rDNA-ITS-PCR
Rhododendron delavayi%mycorrhizal fungi%molecular biology%rDNADNA-ITS-PCR
为探明马缨杜鹃菌根真菌的种类,采用 rDNA-ITS-PCR分子生物学方法对分离的马缨杜鹃菌根真菌进行鉴定。结果表明:从马缨杜鹃菌根共分离出6种与杜鹃花类菌根真菌 Phialocephala sp (CK007137)、Oidiodendron sp(HM208742.1)、Oidiodendron sp.shylhs04(AY268186.1)、2个不同的 Ep-acris root associated fungus 类菌株和 fungal sp.R38亲缘关系十分密切的真菌。其中,前5种真菌与其他ERM 植物的菌根真菌同源或近缘,fungal sp.R38是 ERM 植物菌根真菌的新类型。
為探明馬纓杜鵑菌根真菌的種類,採用 rDNA-ITS-PCR分子生物學方法對分離的馬纓杜鵑菌根真菌進行鑒定。結果錶明:從馬纓杜鵑菌根共分離齣6種與杜鵑花類菌根真菌 Phialocephala sp (CK007137)、Oidiodendron sp(HM208742.1)、Oidiodendron sp.shylhs04(AY268186.1)、2箇不同的 Ep-acris root associated fungus 類菌株和 fungal sp.R38親緣關繫十分密切的真菌。其中,前5種真菌與其他ERM 植物的菌根真菌同源或近緣,fungal sp.R38是 ERM 植物菌根真菌的新類型。
위탐명마영두견균근진균적충류,채용 rDNA-ITS-PCR분자생물학방법대분리적마영두견균근진균진행감정。결과표명:종마영두견균근공분리출6충여두견화류균근진균 Phialocephala sp (CK007137)、Oidiodendron sp(HM208742.1)、Oidiodendron sp.shylhs04(AY268186.1)、2개불동적 Ep-acris root associated fungus 류균주화 fungal sp.R38친연관계십분밀절적진균。기중,전5충진균여기타ERM 식물적균근진균동원혹근연,fungal sp.R38시 ERM 식물균근진균적신류형。
To explore the species of mycorrhizal fungi of R.delavayi,and provide technical reserves for cultivation and application of R.delavayi,the molecular biological method rDNA-ITS-PCR was employed to identify the isolated mycorrhizal fungi of R.delavayi.Results:six kinds of mycorrhizal fungi closely related to ERM were isolated, including Phialocephala sp (CK007137 ), Oidiodendron sp (HM208742.1 ),Oidiodendron sp. shylhs04 (AY268186.1 ), two kinds of different Epacris root associated fungus,which were the relatives of ERM and fungal sp.R38 which was a new type of ERM respectively.