贵州农业科学
貴州農業科學
귀주농업과학
GUIZHOU AGRICULTURAL SCIENCES
2014年
6期
41-43
,共3页
洪军%胡建业%张侠%马肖梦%韩莎
洪軍%鬍建業%張俠%馬肖夢%韓莎
홍군%호건업%장협%마초몽%한사
榴莲果皮%黄酮%抗氧化能力%抗菌活性
榴蓮果皮%黃酮%抗氧化能力%抗菌活性
류련과피%황동%항양화능력%항균활성
durian peel%flavonoids%antioxidant activity%antimicrobial activity
为开发利用榴莲废弃物,采用水杨酸法和 ABTS 法探讨了榴莲果皮中总黄酮对· OH 和ABTS 自由基的抗氧化活性,并通过琼脂平板扩散法测定了黄酮类物质对4种细菌的抗菌活性。结果表明:与 VC 相比,榴莲皮提取的黄酮类物质在质量浓度为0.2~1.0 mg/mL 时,对·OH 和 ABTS 自由基具有较强的清除能力,且在浓度为1.0 mg/mL 时,对·OH 和 ABTS 自由基的清除率分别为25.7%和99.4%;其抑菌活性在榴莲果皮黄酮浓度1.25~10 mg/mL 时对不同细菌的抑菌活性具有一定的差异,金黄色葡萄球菌和绿脓杆菌在其浓度≥1.25 mg/mL 和≥5 mg/mL 时受到明显地抑制,但对大肠杆菌和巨大芽孢杆菌在浓度1.25~10 mg/mL 时无抑菌作用。
為開髮利用榴蓮廢棄物,採用水楊痠法和 ABTS 法探討瞭榴蓮果皮中總黃酮對· OH 和ABTS 自由基的抗氧化活性,併通過瓊脂平闆擴散法測定瞭黃酮類物質對4種細菌的抗菌活性。結果錶明:與 VC 相比,榴蓮皮提取的黃酮類物質在質量濃度為0.2~1.0 mg/mL 時,對·OH 和 ABTS 自由基具有較彊的清除能力,且在濃度為1.0 mg/mL 時,對·OH 和 ABTS 自由基的清除率分彆為25.7%和99.4%;其抑菌活性在榴蓮果皮黃酮濃度1.25~10 mg/mL 時對不同細菌的抑菌活性具有一定的差異,金黃色葡萄毬菌和綠膿桿菌在其濃度≥1.25 mg/mL 和≥5 mg/mL 時受到明顯地抑製,但對大腸桿菌和巨大芽孢桿菌在濃度1.25~10 mg/mL 時無抑菌作用。
위개발이용류련폐기물,채용수양산법화 ABTS 법탐토료류련과피중총황동대· OH 화ABTS 자유기적항양화활성,병통과경지평판확산법측정료황동류물질대4충세균적항균활성。결과표명:여 VC 상비,류련피제취적황동류물질재질량농도위0.2~1.0 mg/mL 시,대·OH 화 ABTS 자유기구유교강적청제능력,차재농도위1.0 mg/mL 시,대·OH 화 ABTS 자유기적청제솔분별위25.7%화99.4%;기억균활성재류련과피황동농도1.25~10 mg/mL 시대불동세균적억균활성구유일정적차이,금황색포도구균화록농간균재기농도≥1.25 mg/mL 화≥5 mg/mL 시수도명현지억제,단대대장간균화거대아포간균재농도1.25~10 mg/mL 시무억균작용。
To develop and utilize durian peels,the scavenging activities of flavonoids extracted from durian peel on · OH and ABTS radicals were detected by salicylic acid and ABTS methods. its antimicrobial activities of flavonoids on four kinds of bacteria were investigated by agar plate diffusion. The results showed that flavonoids from durian peel excelled in scavenging activities on ABTS and ·OH radicals at 0.2~1.0 mg/mL concentrations compared with VC .The flavonoids scavenging abilities on · OH and ABTS+ were 25.7% and 99.4% at 1.0 mg/mL concentration,respectively.Meanwhile,the antibacterial activities assay indicated that flavonoids had different inhibitory effect on different bacteria at 1.25~10 mg/mL concentration. The flavonoids from durian peel had obvious inhibitory effect on Staphylococcus aureus at ≥ 1.25 mg/mL concentration and Pseudomonas aeruginosa at ≥ 5 mg/mL concentration,but they had no effect on Escherichia coli and Bacillus megaterium at 1.25 ~ 10 mg/mL concentration.