西部中医药
西部中醫藥
서부중의약
GANSU JOURNAL OF TRADITIONAL CHINESE MEDICINE
2014年
6期
16-17
,共2页
任一杰%张华%张喜民%邓月婷%贾继禧%刘洁丽
任一傑%張華%張喜民%鄧月婷%賈繼禧%劉潔麗
임일걸%장화%장희민%산월정%가계희%류길려
复方金银花滴丸%质量标准%薄层鉴别%含量测定%绿原酸
複方金銀花滴汍%質量標準%薄層鑒彆%含量測定%綠原痠
복방금은화적환%질량표준%박층감별%함량측정%록원산
compound JinYinHua dripping pills%quality standard%thin-layer chromatography%content determination%chlorogenic acid
目的：建立复方金银花滴丸的质量标准。方法：采用薄层色谱法对处方中黄芩、连翘进行定性鉴别，并应用高效液相色谱法，Hypersil ODS-2(250 mm×4.6 mm，5μm)色谱柱，以乙腈-0.4%磷酸溶液(11∶89)为流动相，检测波长327 nm，流速1.0 mL/min，测定绿原酸的含量。结果：TCL图谱中可检出黄芩、连翘的特征图谱；绿原酸在22.8～57.0μg/mL呈良好线性关系(r=0.9999)，平均加样回收率为101.3%，RSD为1.37%。结论：本法简便、准确，可用于复方金银花滴丸的质量控制。
目的：建立複方金銀花滴汍的質量標準。方法：採用薄層色譜法對處方中黃芩、連翹進行定性鑒彆，併應用高效液相色譜法，Hypersil ODS-2(250 mm×4.6 mm，5μm)色譜柱，以乙腈-0.4%燐痠溶液(11∶89)為流動相，檢測波長327 nm，流速1.0 mL/min，測定綠原痠的含量。結果：TCL圖譜中可檢齣黃芩、連翹的特徵圖譜；綠原痠在22.8～57.0μg/mL呈良好線性關繫(r=0.9999)，平均加樣迴收率為101.3%，RSD為1.37%。結論：本法簡便、準確，可用于複方金銀花滴汍的質量控製。
목적：건립복방금은화적환적질량표준。방법：채용박층색보법대처방중황금、련교진행정성감별，병응용고효액상색보법，Hypersil ODS-2(250 mm×4.6 mm，5μm)색보주，이을정-0.4%린산용액(11∶89)위류동상，검측파장327 nm，류속1.0 mL/min，측정록원산적함량。결과：TCL도보중가검출황금、련교적특정도보；록원산재22.8～57.0μg/mL정량호선성관계(r=0.9999)，평균가양회수솔위101.3%，RSD위1.37%。결론：본법간편、준학，가용우복방금은화적환적질량공제。
Objective: To establish quality standard of compound JinYinHua dripping pills. Methods:HuangQin (Scutellarid baicalensis Georgi) and LianQiao [Forsythia suspense (Thunb.) Vahl] were determined quanti tatively, HPLC was adopted, Hypersil ODS-2 (250 mm ×4.6 mm, 5 μm) chromatographic column, mobile phase:acetonitrile-0.4%phosphoric acid solution(11:89), detection wavelength was 327 nm, flow rate was 1.0 mL/min, the contents of chlorogenic acid were detected. Results:HuangQin and LianQiao could be detected by TLC;chlorogenic acid demonstrated that better linear relationship in the range between 22.8 and 57.0 μg/mL (r=0.999 9), average recovery rate was 101.3%, RSD was 1.37%. Conclusion:The method, simple and accurate, could be used for quality control of compound JinYinHua dripping pills.
