CAJ | 학술논문

目的观察三维适形调强放疗（IMRT）对鼠骨细粒棘球蚴生发细胞生长及凋亡的影响，探讨三维适形调强放疗治疗骨棘球蚴病的临床效果。方法选取骨细粒棘球蚴病大鼠，将骨细粒棘球蚴内囊中的生发细胞进行分离，并进行体外培养，将体外培养成功的骨细粒棘球蚴的生发细胞分为 IMRT放疗组及空白对照组。IMRT放疗组给予总剂量为40 Gy的 IMRT放疗，空白对照组只给予假照射作为阴性对照。于放疗结束24 h后，采用MTT法检测 IMRT对骨细粒棘球蚴生发细胞生长的抑制作用，Hoechst 33258荧光染色观察 IMRT对骨细粒棘球蚴生发细胞凋亡的影响，观察 IMRT对鼠骨细粒棘球蚴病的治疗效果。结果 IMRT作用24 h后，MTT法检测结果可观察到，IMRT放疗组的骨细粒棘球蚴生发细胞吸光度（OD值＝0．213±0．070），远低于空白对照组的（0．792±0．098），差异有统计学意义（P ＜0．05），存在明显的增殖抑制现象；Hoechst 33258荧光染色可观察到 IMRT治疗组的骨细粒棘球蚴生发细胞呈致密浓染的强蓝色荧光，存在明显的核固缩、染色质凝集等细胞凋亡现象，而空白对照组未出现。结论 IMRT对骨细粒棘球蚴生发细胞生长具有明显的抑制作用并可诱导生发细胞凋亡。
목적관찰삼유괄형조강방료（IMRT）대서골세립극구유생발세포생장급조망적영향，탐토삼유괄형조강방료치료골극구유병적림상효과。방법선취골세립극구유병대서，장골세립극구유내낭중적생발세포진행분리，병진행체외배양，장체외배양성공적골세립극구유적생발세포분위 IMRT방료조급공백대조조。IMRT방료조급여총제량위40 Gy적 IMRT방료，공백대조조지급여가조사작위음성대조。우방료결속24 h후，채용MTT법검측 IMRT대골세립극구유생발세포생장적억제작용，Hoechst 33258형광염색관찰 IMRT대골세립극구유생발세포조망적영향，관찰 IMRT대서골세립극구유병적치료효과。결과 IMRT작용24 h후，MTT법검측결과가관찰도，IMRT방료조적골세립극구유생발세포흡광도（OD치＝0．213±0．070），원저우공백대조조적（0．792±0．098），차이유통계학의의（P ＜0．05），존재명현적증식억제현상；Hoechst 33258형광염색가관찰도 IMRT치료조적골세립극구유생발세포정치밀농염적강람색형광，존재명현적핵고축、염색질응집등세포조망현상，이공백대조조미출현。결론 IMRT대골세립극구유생발세포생장구유명현적억제작용병가유도생발세포조망。
Objective To observe the possibility of the growing influence and apoptosis of germinal cells of intensity modulated radiation therapy (IMRT)to the protoscolex of bone echinococcosis in rats,and to in-vestigate the clinical effect of intensity modulated radiation therapy on bone hydatid disease.Methods Purebred bone echinococcosis rats were selected and the germinal cells of bone echinococcosis was separa-ted,and cultured in vitro.The germinal cells of bone echinococcosis was divided into IMRT radiotherapy group and blank control group.IMRT radiotherapy group,giving a total dose of 40 Gy IMRT radiothera-py,blank control group only blank irradiation as a negative control.In radiation therapy after 24 h.And determined by MTT method was used to detect IMRT for growing influence of germinal cells growth inhi-bition,Hoechst 33258 fluorescent staining observation of IMRT for germinal cells apoptosis,in order to observe the clinical effect of intensity modulated radiation therapy on bone hydatid disease.Results 24 hours later,The proliferation of germinal cells of bone echinococcosis cells was significantly inhibited by MTT assay in the IMRT group.Distinct morphologic changes of cell apoptosis such as karyopyknosis and chromatic agglutination were observed by Hoechst 33258 staining.Conclusion It is evident that the IMRT can promote the growing influence and apoptosis of germinal cells of bone echinococcosis.