CAJ | 학술논문

目的：探讨宫颈人乳头瘤病毒（HPV）感染后患者血清内炎症相关microRNA表达变化及其临床意义。方法：收集行宫颈筛查妇女，经HPV-DNA分析和病理组织学检查，选取14例高危型HPV感染宫颈上皮内瘤变I（ CIN I）患者、10例高危型HPV感染CIN Ⅱ／Ⅲ患者、65例宫颈炎伴高危型HPV感染者以及25例无HPV感染宫颈炎患者，采集外周静脉血3ml。1年后对以上90例宫颈炎病人复检宫颈HPV-DNA和病理类型，同时采集外周静脉血3ml。应用实时定量PCR方法分析以上血清内5个炎症相关microRNA （miR-21、miR-31、miR-125b、miR-146a和miR-155）随HPV感染后宫颈病变进展的变化。结果：65例高危型HPV感染宫颈炎病人中，52．3％（34／65）的感染者在1年后HPV感染消失（一过性感染），而35．4％（23／65）的感染者在1年后仍存在同型的HPV感染（持续性感染）。一过性感染患者血清内 miR-21水平与未感染者相似，但持续感染者、CIN I、CIN Ⅱ／Ⅲ患者血清内该分子表达均上调。血清内miR-146a分子表达水平随 HPV 感染后宫颈病变发展而升高，一过性感染病人较未感染病人高，持续感染病人较一过性感染高，而CIN Ⅱ／Ⅲ病人又较宫颈炎病人升高。结论：血清内miR-21和miR-146a可以作为预测 HPV感染后宫颈病变进展情况的生物标记物。
목적：탐토궁경인유두류병독（HPV）감염후환자혈청내염증상관microRNA표체변화급기림상의의。방법：수집행궁경사사부녀，경HPV-DNA분석화병리조직학검사，선취14례고위형HPV감염궁경상피내류변I（ CIN I）환자、10례고위형HPV감염CIN Ⅱ／Ⅲ환자、65례궁경염반고위형HPV감염자이급25례무HPV감염궁경염환자，채집외주정맥혈3ml。1년후대이상90례궁경염병인복검궁경HPV-DNA화병리류형，동시채집외주정맥혈3ml。응용실시정량PCR방법분석이상혈청내5개염증상관microRNA （miR-21、miR-31、miR-125b、miR-146a화miR-155）수HPV감염후궁경병변진전적변화。결과：65례고위형HPV감염궁경염병인중，52．3％（34／65）적감염자재1년후HPV감염소실（일과성감염），이35．4％（23／65）적감염자재1년후잉존재동형적HPV감염（지속성감염）。일과성감염환자혈청내 miR-21수평여미감염자상사，단지속감염자、CIN I、CIN Ⅱ／Ⅲ환자혈청내해분자표체균상조。혈청내miR-146a분자표체수평수 HPV 감염후궁경병변발전이승고，일과성감염병인교미감염병인고，지속감염병인교일과성감염고，이CIN Ⅱ／Ⅲ병인우교궁경염병인승고。결론：혈청내miR-21화miR-146a가이작위예측 HPV감염후궁경병변진전정황적생물표기물。
Objective:To study the expression and clinical significance of serum inflammation-related mi-croRNA in cervical lesions caused by human papillomavirus infection (HPV) .Methods :We quantified five serum in-flammation related microRNAs (miR-21 ,miR-31 ,miR-125b ,miR-146a and miR-155) in 14 women with HPV in-fection and Cervical Intraepithelial Neoplasia (CIN I) ,10 with HPV infection and CIN Ⅱ /Ⅲ ,65 with HPV infec-tion and cervicitis ,and 25 with cervicitis but without HPV infection using real time PCR .These 90 patients with cer-vicitis were tested for HPV-DNA genotype ,pathological feature ,and serum miRNA levels one year later .Results :Among these 65 patients initially with HPV and cervicitis ,the test one year later revealed that 52 .3% subjects did not have HPV infection (transient infection ,TI) ,and 35 .4% still had infection with the same genotype HPV (per-sistent infection ,PI) .Compared with the patients without HPV infection ,TI patients had a similar miR-21 level in serum ,whereas ,the patients with PI ,CIN I ,or CIN Ⅱ /Ⅲ displayed significantly elevated serum miR-21 expres-sion .Moreover ,serum miR-146a increased along with the HPV infected cervical lesion progressed ,TI patients >patients without infection ,PI > TI ,CIN Ⅱ /Ⅲ > cervicitis Conclusion :miR-21 and miR-146a in serum can be used as biomarkers to predict the outcome or progress of cervical lesions caused by HPV infection .