CAJ | 학술논문

目的：建立牛至中迷迭香酸含量的 HPLC 测定方法。方法采用高效液相色谱法（HPLC）测定牛至中迷迭香酸的含量。色谱条件：Kromasil C18柱（4．6 mm ×150 mm，5μm），流动相为甲醇-1．5％乙酸水（38∶62），流速为1．0 mL／min，检测波长为330 nm，柱温25℃。结果迷迭香酸峰面积与进样量在0．3321～1．6605μg 范围内有良好的线性关系（r ＝0．9999），平均回收率为98．96％，RSD 为1．09％（n ＝6）。10个产地的牛至中迷迭香酸含量为8．13～15．74 mg／g。结论HPLC 法操作简单、准确可靠，可用于牛至中迷迭香酸含量的测定。
목적：건립우지중미질향산함량적 HPLC 측정방법。방법채용고효액상색보법（HPLC）측정우지중미질향산적함량。색보조건：Kromasil C18주（4．6 mm ×150 mm，5μm），류동상위갑순-1．5％을산수（38∶62），류속위1．0 mL／min，검측파장위330 nm，주온25℃。결과미질향산봉면적여진양량재0．3321～1．6605μg 범위내유량호적선성관계（r ＝0．9999），평균회수솔위98．96％，RSD 위1．09％（n ＝6）。10개산지적우지중미질향산함량위8．13～15．74 mg／g。결론HPLC 법조작간단、준학가고，가용우우지중미질향산함량적측정。
Objective To establish a method for the content determination of Rosmarinic acid inOriganum vulgare L..Methods The content of Rosmarinic acid was detected by HPLC.The separation was carried out on a Kromasil C18 column (150 mm×4.6 mm,5 μm).The mobile phase was Methanol-1.5% Acetic acid solution (38∶62);the flow rate was 1.0 mL/min;the detective wavelength was set at 330 nm.The column temperature was 25 ℃.Results The calibration curve showed good linearity over the range of 0.332 1-1.660 5 μg (r =0.999 9).The average recovery was 98.96% with RSD 1.09% (n =6).The content of Rosmarinic acid in Origanum vulgare L.from 10 different regions were between to 8.13-15.74 mg/g. Conclusion This method was rapid,accurate,reliable;it could be used as a determination of Rosmarinic acid in Origanum vulgare L..