安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2014年
7期
995-998,999
,共5页
THBS2%胃癌%预后因子
THBS2%胃癌%預後因子
THBS2%위암%예후인자
THBS2%gastric cancer%prognostic biomarker
目的：探讨胃癌中血小板反应蛋白2( THBS2)的表达及其临床意义。方法在14对配对胃癌样本及其正常对照中，使用实时荧光定量PCR和Western blot法检测THBS2 mRNA和蛋白表达水平。使用129例胃癌样本构成的组织芯片评价THBS2的临床意义。生存分析使用Kaplan-Meier法和Cox比例风险模型。结果 THBS2 mRNA和蛋白表达在14对样本中有12例胃癌样本中都显著低于其正常对照。与Western blot结果一致，组织芯片中胃癌THBS2表达明显低于正常对照(P=0．031)。高表达的THBS2与患者的良好预后密切相关( P=0．002)，并且THBS2表达越低胃癌的组织分化程度越低( P =0．005)。结论与正常胃组织相比THBS2在胃癌组织中表达下调，可能在胃癌的发生发展中起到重要的作用，并且可作为一个潜在的胃癌临床预后因子。
目的：探討胃癌中血小闆反應蛋白2( THBS2)的錶達及其臨床意義。方法在14對配對胃癌樣本及其正常對照中，使用實時熒光定量PCR和Western blot法檢測THBS2 mRNA和蛋白錶達水平。使用129例胃癌樣本構成的組織芯片評價THBS2的臨床意義。生存分析使用Kaplan-Meier法和Cox比例風險模型。結果 THBS2 mRNA和蛋白錶達在14對樣本中有12例胃癌樣本中都顯著低于其正常對照。與Western blot結果一緻，組織芯片中胃癌THBS2錶達明顯低于正常對照(P=0．031)。高錶達的THBS2與患者的良好預後密切相關( P=0．002)，併且THBS2錶達越低胃癌的組織分化程度越低( P =0．005)。結論與正常胃組織相比THBS2在胃癌組織中錶達下調，可能在胃癌的髮生髮展中起到重要的作用，併且可作為一箇潛在的胃癌臨床預後因子。
목적：탐토위암중혈소판반응단백2( THBS2)적표체급기림상의의。방법재14대배대위암양본급기정상대조중，사용실시형광정량PCR화Western blot법검측THBS2 mRNA화단백표체수평。사용129례위암양본구성적조직심편평개THBS2적림상의의。생존분석사용Kaplan-Meier법화Cox비례풍험모형。결과 THBS2 mRNA화단백표체재14대양본중유12례위암양본중도현저저우기정상대조。여Western blot결과일치，조직심편중위암THBS2표체명현저우정상대조(P=0．031)。고표체적THBS2여환자적량호예후밀절상관( P=0．002)，병차THBS2표체월저위암적조직분화정도월저( P =0．005)。결론여정상위조직상비THBS2재위암조직중표체하조，가능재위암적발생발전중기도중요적작용，병차가작위일개잠재적위암림상예후인자。
Objective To explore the expression of THBS 2 and its clinical significance in gastric cancer . Methods The mRNA and protein expression levels of THBS2 were assessed in 14 paired of gastric cancer specimen and corre-sponding normal mucosa using quantitative real-time PCR and Western blot analysis. Immunohistochemistry of TH-BS2 on a population-based tissue microarray consisting of 129 gastric cancer cases was used for evaluating the TH-BS2 prognostic significance. Kaplan-Meier method and Cox′s proportional hazards model were used in survival anal-ysis. Results Both mRNA and protein expression of THBS2 in 12 gastric cancer tissues were remarkably lower than the corresponding normal tissues among total 14 pair tissues. Consistent with the results of our Western blot, THBS2 expression was significantly inhibited in gastric cancer tissues as that in the normal controls in TMA ( P=0. 031) . Overexpression of THBS2 had a significant correlation with favorable prognosis of gastric cancer patients ( P=0. 002 ) and decreasing THBS2 expression was associated with the poor histological grade of gastric cancer his-tological grade ( P=0. 005 ) . Conclusion This study suggests THBS2 is down-regulation in gastric cancer tissue versus normal gastric tissue, and it may play a critical role in gastric cancer carcinogenesis and may be a potential prognosis predictor of gastric cancer.
