CAJ | 학술논문

目的探讨人脑胶质组织中EDNRB基因的甲基化状态及其蛋白表达的关系。方法采用巢式PCR、亚硫酸氢盐测序PCR( BSP)及免疫组织化学技术方法，检测50例脑胶质瘤组织标本中EDNRB基因启动子区甲基化状态，分析其甲基化水平、蛋白表达和临床资料之间的相关性。结果脑胶质瘤组织中 EDNRB 基因启动子区甲基化率为38%(19/50)，其中低级别组(Ⅰ级~Ⅱ级)中EDNRB基因甲基化率为26.5%(9/34)，高级别组(Ⅲ级~Ⅳ级)EDNRB基因甲基化率为62.5%(10/16)，两组比较差异有统计学意义(P<0．05)。而其中甲基化蛋白表达阳性率为36．8%(7/19)，未甲基化的蛋白表达阳性率为71%(22/31)。结论组织中的EDNRB基因启动子区CpG岛高甲基化与胶质瘤的发病有关，与胶质瘤的病理级别关系密切，而与患者年龄、性别、肿瘤分型无关；基因甲基化率抑制其蛋白表达。
목적탐토인뇌효질조직중EDNRB기인적갑기화상태급기단백표체적관계。방법채용소식PCR、아류산경염측서PCR( BSP)급면역조직화학기술방법，검측50례뇌효질류조직표본중EDNRB기인계동자구갑기화상태，분석기갑기화수평、단백표체화림상자료지간적상관성。결과뇌효질류조직중 EDNRB 기인계동자구갑기화솔위38%(19/50)，기중저급별조(Ⅰ급~Ⅱ급)중EDNRB기인갑기화솔위26.5%(9/34)，고급별조(Ⅲ급~Ⅳ급)EDNRB기인갑기화솔위62.5%(10/16)，량조비교차이유통계학의의(P<0．05)。이기중갑기화단백표체양성솔위36．8%(7/19)，미갑기화적단백표체양성솔위71%(22/31)。결론조직중적EDNRB기인계동자구CpG도고갑기화여효질류적발병유관，여효질류적병리급별관계밀절，이여환자년령、성별、종류분형무관；기인갑기화솔억제기단백표체。
Abstarct Objective To explore the relation between methylation of EDNRB gene and its protein expression in hu-man brain glial tissues. Methods By using nest PCR, bisulfite PCR and immunohistochemistry staining, we in-vestigated the methylation status at EDNRB gene promoter, EDNRB protein expression and their association with patient clinical history. Results In this study, we found EDNRB gene promoter methylation among 38% of total glioma samples(19/50), with 26. 5% among low grade glioma while 62. 5% among high grade gliomas, which was statistically significant. Within the samples with EDNRB gene methylation events detected, 36. 8% of these sam-ples showed protein expression detected by IHC while 71% samples without EDNRB gene methylation were IHC positive. Conclusion Our study supports the EDNRB gene promoter methylation contributes to gliomagenesis. Mo-reover, the methylation status correlates with grade of glioma but not with patient age, gender, tumor subtype. The methylation status is negative related to protein expression in gliomas.