CAJ | 학술논문

目的：检测基质金属蛋白酶-26( MMP-26)在人胰腺癌中的表达并探讨其在胰腺癌进展中的作用及意义。方法通过免疫组化方法、逆转录聚合酶链反应( RT-PCR)和蛋白质印记( Western blot)分别检测MMP-26基因和蛋白在胰腺癌、癌旁正常组织中的表达，并探讨其与胰腺癌临床病理特征之间的关系。结果免疫组化显示MMP-26蛋白在胰腺癌中的阳性表达率(72%)比癌旁正常组织中(6%)明显升高，差异有统计学意义( P<0．05)；其高表达与胰腺癌的分化程度、TNM分期以及淋巴结转移有关( P<0．05)。 RT-PCR检测胰腺癌组织及癌旁正常组织各10例，有7例(70%)胰腺癌组织MMP-26 mRNA的表达高于癌旁正常组织。 Western blot法检测胰腺癌组织及癌旁正常组织各7例，发现有5例(71．4%)胰腺癌组织MMP-26蛋白的表达高于癌旁正常组织。结论 MMP-26的表达与胰腺癌的分化程度、TNM分期以及淋巴结转移均有相关性，有可能成为胰腺癌早期诊断的一个生物标志物及临床治疗的新靶点。
목적：검측기질금속단백매-26( MMP-26)재인이선암중적표체병탐토기재이선암진전중적작용급의의。방법통과면역조화방법、역전록취합매련반응( RT-PCR)화단백질인기( Western blot)분별검측MMP-26기인화단백재이선암、암방정상조직중적표체，병탐토기여이선암림상병리특정지간적관계。결과면역조화현시MMP-26단백재이선암중적양성표체솔(72%)비암방정상조직중(6%)명현승고，차이유통계학의의( P<0．05)；기고표체여이선암적분화정도、TNM분기이급림파결전이유관( P<0．05)。 RT-PCR검측이선암조직급암방정상조직각10례，유7례(70%)이선암조직MMP-26 mRNA적표체고우암방정상조직。 Western blot법검측이선암조직급암방정상조직각7례，발현유5례(71．4%)이선암조직MMP-26단백적표체고우암방정상조직。결론 MMP-26적표체여이선암적분화정도、TNM분기이급림파결전이균유상관성，유가능성위이선암조기진단적일개생물표지물급림상치료적신파점。
Objective To detect MMP-26 expression in human pancreatic cancer and to investigate its role and sig-nificance in the progression of pancreatic cancer. Methods Immunohistochemistry, RT-PCR and Western blot were used to detect MMP-26 mRNA and protein in pancreatic cancer and adjacent normal tissue, to investigate the relationship between the clinical and pathological features of pancreatic cancer with MMP-26 . Results The posi-tive expression rate of MMP-26 protein was significantly higher in pancreatic cancer ( 72%) than in adjacent nor-mal tissues (6%) by immunohistochemical method, difference was considered statistically significant (P<0. 05). The high expression of MMP-26 was correlated with pancreatic differentiation, TNM stage and lymph node metasta-sis. RT-PCR detected 10 cases of pancreatic cancer tissues and adjacent normal tissues respectively, in which 7 ca-ses (70%) were discovered the positive expression of MMP-26 mRNA was significantly unregulated in pancreatic tissue than in adjacent normal tissues. Western blot detected 7 cases of pancreatic cancer tissues and adjacent nor-mal tissues respectively, in which 5 cases (71. 4%) were discovered the positive expression of MMP-26 mRNA was significantly higher in pancreatic tissue than in adjacent normal tissue. Conclusion The expression of MMP-26 is correlated with pancreatic differentiation, TNM stage and lymph node metastasis, may become a novel biomarker and clinical therapeutic target of pancreatic cancer.