CAJ | 학술논문

目的：通过系统性红斑狼疮( SLE)患者外周血单个核细胞( PBMCs)的磷蛋白组学分析，为SLE的进一步机制研究及治疗奠定基础。方法收集15例SLE患者和15例健康受试者的外周血，使用 TiO2富集 PBMCs 的磷酸化肽段，进行质谱分析，然后进行磷酸化肽段和磷酸化位点鉴定，并进行生物信息学分析。结果 SLE患者与正常人存在有差异的1035个磷酸化位点，与标注蛋白对应的基因有618个。共筛选出12条代谢通路，其中丝裂原活化蛋白激酶( MAPKs)通路含差异的磷酸化位点最多。结论 SLE患者PBMCs具有差异的磷酸化蛋白质及肽段，与代谢通路一起可作为SLE发病机制研究参考和补充，并可作为治疗靶点研究。
목적：통과계통성홍반랑창( SLE)환자외주혈단개핵세포( PBMCs)적린단백조학분석，위SLE적진일보궤제연구급치료전정기출。방법수집15례SLE환자화15례건강수시자적외주혈，사용 TiO2부집 PBMCs 적린산화태단，진행질보분석，연후진행린산화태단화린산화위점감정，병진행생물신식학분석。결과 SLE환자여정상인존재유차이적1035개린산화위점，여표주단백대응적기인유618개。공사선출12조대사통로，기중사렬원활화단백격매( MAPKs)통로함차이적린산화위점최다。결론 SLE환자PBMCs구유차이적린산화단백질급태단，여대사통로일기가작위SLE발병궤제연구삼고화보충，병가작위치료파점연구。
Objective To investigate the aberrant expression of phosphoproteome analysis of peripheral blood mononuclear cells( PBMCs) in patients with systemic lupus erythematosus( SLE) . Lay the foundation for further re-search of mechanism and treatment in patients with SLE. Methods Phosphopeptides were enriched using TiO2 from PBMCs of patients and healthy subjects, then analyzed by automated LC-MS/MS analysis. Phosphorylation sites were identified and quantitated by MASCOT and MaxQuant. Differential expressed proteins and peptides were screened based on the bioinformatics analysis. Results 1 035 phosphorylation sites were identified from SLE com-prared with normal subjects, 618 corresponding genes were screened out in annotation proteins. Pathway analyses showed 12 signaling pathways were identified. There were the most difference phosphorylation sites in mitogen acti-vated protein kinases( MAPK) signaling pathway. Conclusion Differently phosphorylated proteins and peptides can be detected in patients with SLE, which can be used as a mechanism of reference and supplement combined with metabolic pathway, and might be used as a potential target for treatment and research of SLE.