CAJ | 학술논문

目的探讨微RNA-145(miR-145)对自体静脉桥内膜增生的影响。方法18只雄性SD大鼠均分为慢病毒处理组、慢病毒对照组和未处理组。移植前,慢病毒处理组将移植静脉浸入慢病毒介导的miR-145溶液中(病毒滴度为1．0×109 pfu/ml)15 min,慢病毒对照组将移植静脉浸入空载慢病毒溶液(病毒滴度为1．0×109 pfu/ml)浸泡15 min,未处理组在生理盐水中浸泡15 min。术后2周取出移植血管,应用HE染色和Masson染色观察血管内膜的病理学改变,以及荧光定量 RT-PCR 法检测移植血管中单核细胞趋化蛋白-1(MCP-1)、增殖细胞核抗原(PCNA)、α-平滑肌肌动蛋白(α-SMA)及血管内皮生长因子( VEGF)的表达。结果慢病毒处理组移植血管内膜厚度和管腔狭窄度均较慢病毒对照组和未处理组明显减少, MCP-1、PCNA、α-SMA及 VEGF的表达水平明显低于慢病毒对照组和未处理组。结论 miR-145可以有效抑制移植静脉内膜的增生。
목적탐토미RNA-145(miR-145)대자체정맥교내막증생적영향。방법18지웅성SD대서균분위만병독처리조、만병독대조조화미처리조。이식전,만병독처리조장이식정맥침입만병독개도적miR-145용액중(병독적도위1．0×109 pfu/ml)15 min,만병독대조조장이식정맥침입공재만병독용액(병독적도위1．0×109 pfu/ml)침포15 min,미처리조재생리염수중침포15 min。술후2주취출이식혈관,응용HE염색화Masson염색관찰혈관내막적병이학개변,이급형광정량 RT-PCR 법검측이식혈관중단핵세포추화단백-1(MCP-1)、증식세포핵항원(PCNA)、α-평활기기동단백(α-SMA)급혈관내피생장인자( VEGF)적표체。결과만병독처리조이식혈관내막후도화관강협착도균교만병독대조조화미처리조명현감소, MCP-1、PCNA、α-SMA급 VEGF적표체수평명현저우만병독대조조화미처리조。결론 miR-145가이유효억제이식정맥내막적증생。
Objective To study the function of miR-145 on intimal hyperplasia in autogenous vein grafts rats. Methods Eighteen male SD rats were divided into three groups: lentivirus treatment group, lentivirus control group and untreated group. Every group has six rats. Before transplantation, the graft which was from lentivirus treatment group was treated in a solution of miR-145 lentivirus ( virus titer was 1 × 109 pfu/ml) at 15 min, the graft which was from lentivirus control group was treated in a solution of lentivirus ( virus titer was 1 × 109 pfu/ml) at 15 min, the untreated group was treated in saline at 15 min. The vein grafts were collected two weeks after transplan-ted, pathological changes of intimal hyperplasia were detected by HE and Masson staining. The expression of mono-cyte chemotactic protein-1(MCP-1),proliferating cell nuclear antigen (PCNA),α-smooth muscle actin (α-SMA) and vascular endothelial growth factor ( VEGF) in vein graft were detected by fluorogenetic quantitation RT-PCR. Results The thickness of intimal and the degrees of restenosis of lumen in lentivirus treatment group were signifi-cantly lower than that in lentivirus control group and untreated group, the expression level of MCP-1, PCNA, α -SMA and VEGF was also significantly lower than that in the lentivirus control group and untreated group. Conclu-sion miR-145 can effectively inhibit the proliferation hyperplasia of vein graft.