CAJ | 학술논문

目的研究全反式维甲酸( ATRA)对人胃腺癌细胞株BGC-823细胞迁移能力的影响及其可能机制。方法不同浓度ATRA处理BGC-823后,MTT法检测ATRA对细胞增殖的影响并计算半数抑制率( IC50),倒置显微镜下观察细胞形态学变化,划痕法检测其对细胞迁移能力的作用,平板克隆法观察其对细胞体外克隆形成能力的影响, Western blot法检测可能与迁移相关蛋白的表达。结果 ATRA对BGC-823细胞增殖具有明显的抑制作用,呈浓度依赖性, IC50为42．4025μmol/L；ATRA可抑制细胞生长速度,使细胞形态拉长；ATRA可抑制BGC-823细胞的迁移；ATRA可明显降低细胞克隆形成数(P<0．05)；Western blot结果显示ATRA显著降低肌球蛋白轻链激酶( MLCK)的表达和肌球蛋白轻链( MLC)的磷酸化。结论 ATRA 对人胃腺癌细胞株 BGC-823细胞的迁移具有抑制作用,可能与降低 MLCK 表达和MLC磷酸化水平有关。
목적연구전반식유갑산( ATRA)대인위선암세포주BGC-823세포천이능력적영향급기가능궤제。방법불동농도ATRA처리BGC-823후,MTT법검측ATRA대세포증식적영향병계산반수억제솔( IC50),도치현미경하관찰세포형태학변화,화흔법검측기대세포천이능력적작용,평판극륭법관찰기대세포체외극륭형성능력적영향, Western blot법검측가능여천이상관단백적표체。결과 ATRA대BGC-823세포증식구유명현적억제작용,정농도의뢰성, IC50위42．4025μmol/L；ATRA가억제세포생장속도,사세포형태랍장；ATRA가억제BGC-823세포적천이；ATRA가명현강저세포극륭형성수(P<0．05)；Western blot결과현시ATRA현저강저기구단백경련격매( MLCK)적표체화기구단백경련( MLC)적린산화。결론 ATRA 대인위선암세포주 BGC-823세포적천이구유억제작용,가능여강저 MLCK 표체화MLC린산화수평유관。
Objective To explore the effect of all-trans retinoic acid ( ATRA ) on the migration of human gastric cancer BGC-823 cells and its probable mechanism. Methods MTT assay was performed to measure the prolifera-tion of BGC-823 cells treated with different concentrations of ATRA and calculated the IC50 . Light microscope was used to observe morphologic changes. The effect of ATRA on the migration of BGC-823 cells was analyzed by wound healing assay. The effect of ATRA on the colony formation rate of BGC-823 cells was measured by plate col-ony formation assay. The expression of proteins involved in migration was detected by Western blot. Results AT-RA could inhibit the proliferation of cell line BGC-823 significantly. With the increase of concentration,the inhibi-tion rate was more obvious and the IC50 was 42. 402 5 μmol/L. ATRA could inhibit cell growth and made them e-longated. ATRA could decrease the distances of migration of BGC-823. ATRA could decrease the colony formation of BGC-823 significantly, compared with DMSO control(P<0. 05). ATRA could inhibit the expression of MLCK and phosphorylation of MLC protein which was detected by Western blot. Conclusion ATRA can inhibit the mi-gration of human gastric carcinoma cell line BGC-823,which may be related to the down regulation of expression of MLCK and phosphorylation of MLC protein.