CAJ | 학술논문

目的：探讨膜攻击复合物C5b-9在大鼠肝脏缺血再灌注损伤中的表达。方法健康雄性SD大鼠60只，180～200 g，随机分为5组（n＝12）：假手术组（S组）、全肝再灌组1 h组（I/R 1 h组）、3 h组（I/R 3 h组）、6 h组（I/R 6 h组）和24 h组（I/R 24 h组）。制备大鼠全肝缺血再灌注模型，再灌注1、3、6、24 h末，取血测定谷丙转氨酶（ALT）和总胆红素（TBIL）浓度。取肝脏组织，检测C5b-9 mRNA和C5b-9蛋白的表达。结果与对照组比较，I/R各组大鼠ALT和TBIL水平均明显升高，I/R 3 h组达到高峰（P＜0.05）；I/R各组C5b-9的mRNA表达和C5b-9蛋白含量均有显著升高，且随着缺血再灌注时间的延长而逐渐升高，其中I/R 24 h组最高（P＜0.05）。结论补体C5b-9参与肝缺血再灌注的损伤，尤其是在再灌注24 h时达到高峰。
목적：탐토막공격복합물C5b-9재대서간장결혈재관주손상중적표체。방법건강웅성SD대서60지，180～200 g，수궤분위5조（n＝12）：가수술조（S조）、전간재관조1 h조（I/R 1 h조）、3 h조（I/R 3 h조）、6 h조（I/R 6 h조）화24 h조（I/R 24 h조）。제비대서전간결혈재관주모형，재관주1、3、6、24 h말，취혈측정곡병전안매（ALT）화총담홍소（TBIL）농도。취간장조직，검측C5b-9 mRNA화C5b-9단백적표체。결과여대조조비교，I/R각조대서ALT화TBIL수평균명현승고，I/R 3 h조체도고봉（P＜0.05）；I/R각조C5b-9적mRNA표체화C5b-9단백함량균유현저승고，차수착결혈재관주시간적연장이축점승고，기중I/R 24 h조최고（P＜0.05）。결론보체C5b-9삼여간결혈재관주적손상，우기시재재관주24 h시체도고봉。
ObjectiveTo investigate the expression of the membrane attack complex C5b-9 on total hepatic ischemia-reperfusion injury in rats.MethodsSixty male healthy SD rats weighing 180-200 g were randomly divided into 5 groups: control group (S group) and 1 h, 3 h, 6 h and 24 h hepatic ischemia-reperfusion group(I/R 1 h, 3 h, 6 h and 24 h,n=12). The hepatic I/R model was reproduced, and the concentration of alanine aminotransferase (ALT) and total bilirubin (TBIL) in blood was determined respectively in end of reperfusion for 1, 3, 6, 24 h; Hepatic tissues were obtained and the expression of C5b-9 gene and C5b-9 protein involved in all groups were detected.ResultsCompared with control group, the concentration of ALT and TBIL was increased significantly in all I/R groups (P<0.05), which was increased to the peak in I/R 3 h group. The expression of C5b-9 mRNA and protein detected in all I/R groups were higher than those of the control group (P<0.05) and were peaked in I/R 24 h group. ConclusionActivation of C5b-9 was one of the mechanisms in hepatic ischemia reperfusion injury, especially in the reperfusion for 24 hours.