中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2014年
12期
2250-2255
,共6页
青蒿素%哮喘%T淋巴细胞,调节性%CD4+ T淋巴细胞%Th17%肺功能
青蒿素%哮喘%T淋巴細胞,調節性%CD4+ T淋巴細胞%Th17%肺功能
청호소%효천%T림파세포,조절성%CD4+ T림파세포%Th17%폐공능
ARTEMISININ%Asthma%T-lymphocytes,regulatory%CD4+ T lymphocyte%Th17%Pulmonary function
目的：探讨青蒿素对支气管哮喘患者外周血Treg/Th17表达的调控机制。方法将随访的145例哮喘患者按照ACT评分分成完全控制组、部分控制组、未控制组，再从每组中按性别、年龄、BMI匹配随机分别抽取20例、21例、7例作为研究对象，入选同期于医院就诊的哮喘急性发作期患者18例和医院体检的健康成人19例作为对照组。流式细胞术检测各组CD4+T淋巴细胞Treg和Th17的表达，将CD4+T淋巴细胞用一定浓度的青蒿素干预共培养24 h检测对比。结果未控制组及急性发作期哮喘患者外周血CD4+T淋巴细胞Th17比例明显高于正常对照组、完全控制组及部分控制组哮喘患者（P＜0.05），Treg比例明显低于正常对照组、完全控制组及部分控制组（P＜0.05）；完全控制组及部分控制组哮喘患者外周血CD4+T淋巴细胞Treg比例稍低于正常对照组（P＜0.05），而Th17比例较正常组差异无统计学意义（P＞0.05）；与青蒿素共浴培养24 h后，未控制组哮喘患者CD4+T淋巴细胞中Th17较干预前明显减低（P＜0.05），Treg较干预前明显升高（P＜0.05）；急性发作组哮喘患者Th17较干预前明显减低（P＜0.05），Treg较干预前明显升高（P＜0.05）；部分控制组哮喘患者Th17较干预前差异无统计学意义（P＞0.05），Treg较干预前差异无统计学意义（P＞0.05）；完全控制组哮喘患者 Th17较干预前稍减低（P＜0.05），Treg 较干预前差异无统计学意义（P＞0.05）。结论青蒿素在体外对支气管哮喘，尤其是难治性哮喘的外周血CD4+ T淋巴细胞中 Treg/Th17有一定的调控作用，并在一定程度上可改善难治性哮喘患者 CD4+ T 淋巴细胞中Treg/Th17免疫失衡。
目的：探討青蒿素對支氣管哮喘患者外週血Treg/Th17錶達的調控機製。方法將隨訪的145例哮喘患者按照ACT評分分成完全控製組、部分控製組、未控製組，再從每組中按性彆、年齡、BMI匹配隨機分彆抽取20例、21例、7例作為研究對象，入選同期于醫院就診的哮喘急性髮作期患者18例和醫院體檢的健康成人19例作為對照組。流式細胞術檢測各組CD4+T淋巴細胞Treg和Th17的錶達，將CD4+T淋巴細胞用一定濃度的青蒿素榦預共培養24 h檢測對比。結果未控製組及急性髮作期哮喘患者外週血CD4+T淋巴細胞Th17比例明顯高于正常對照組、完全控製組及部分控製組哮喘患者（P＜0.05），Treg比例明顯低于正常對照組、完全控製組及部分控製組（P＜0.05）；完全控製組及部分控製組哮喘患者外週血CD4+T淋巴細胞Treg比例稍低于正常對照組（P＜0.05），而Th17比例較正常組差異無統計學意義（P＞0.05）；與青蒿素共浴培養24 h後，未控製組哮喘患者CD4+T淋巴細胞中Th17較榦預前明顯減低（P＜0.05），Treg較榦預前明顯升高（P＜0.05）；急性髮作組哮喘患者Th17較榦預前明顯減低（P＜0.05），Treg較榦預前明顯升高（P＜0.05）；部分控製組哮喘患者Th17較榦預前差異無統計學意義（P＞0.05），Treg較榦預前差異無統計學意義（P＞0.05）；完全控製組哮喘患者 Th17較榦預前稍減低（P＜0.05），Treg 較榦預前差異無統計學意義（P＞0.05）。結論青蒿素在體外對支氣管哮喘，尤其是難治性哮喘的外週血CD4+ T淋巴細胞中 Treg/Th17有一定的調控作用，併在一定程度上可改善難治性哮喘患者 CD4+ T 淋巴細胞中Treg/Th17免疫失衡。
목적：탐토청호소대지기관효천환자외주혈Treg/Th17표체적조공궤제。방법장수방적145례효천환자안조ACT평분분성완전공제조、부분공제조、미공제조，재종매조중안성별、년령、BMI필배수궤분별추취20례、21례、7례작위연구대상，입선동기우의원취진적효천급성발작기환자18례화의원체검적건강성인19례작위대조조。류식세포술검측각조CD4+T림파세포Treg화Th17적표체，장CD4+T림파세포용일정농도적청호소간예공배양24 h검측대비。결과미공제조급급성발작기효천환자외주혈CD4+T림파세포Th17비례명현고우정상대조조、완전공제조급부분공제조효천환자（P＜0.05），Treg비례명현저우정상대조조、완전공제조급부분공제조（P＜0.05）；완전공제조급부분공제조효천환자외주혈CD4+T림파세포Treg비례초저우정상대조조（P＜0.05），이Th17비례교정상조차이무통계학의의（P＞0.05）；여청호소공욕배양24 h후，미공제조효천환자CD4+T림파세포중Th17교간예전명현감저（P＜0.05），Treg교간예전명현승고（P＜0.05）；급성발작조효천환자Th17교간예전명현감저（P＜0.05），Treg교간예전명현승고（P＜0.05）；부분공제조효천환자Th17교간예전차이무통계학의의（P＞0.05），Treg교간예전차이무통계학의의（P＞0.05）；완전공제조효천환자 Th17교간예전초감저（P＜0.05），Treg 교간예전차이무통계학의의（P＞0.05）。결론청호소재체외대지기관효천，우기시난치성효천적외주혈CD4+ T림파세포중 Treg/Th17유일정적조공작용，병재일정정도상가개선난치성효천환자 CD4+ T 림파세포중Treg/Th17면역실형。
Objective To detectin vitrostudy in the regulation of artemisinin on bronchial asthma of peripheral blood Th17/Treg. MethodsAccording to the asthma control test (ACT) scores, followed-up 145 patients with asthma were divided into controlled, partly controlled and uncontrolled group, then which were selected randomly 20, 21, and 7 cases as the study ones by sex, age and BMI matching, meanwhile 18 patients with acute exacerbation of asthma and 19 healthy adults as the control. Artemisinin and CD4+T lymphocytes were co-cultured for 24 hours, used flow cytometry to detect the expression of Th17 and Treg and compared with no co-cultured. ResultsTh17 ratio in CD4+T lymphocytes of the peripheral blood in uncontrolled group and acute exacerbation of asthma was significantly higher than that of other groups(P<0.05), and Treg ratio in which was significantly lower than that of other groups(P<0.05). Meanwhile, Treg ratio in other asthmatic groups was slightly lower than that of normal group (P<0.05). After Artemisinin and CD4+T lymphocytes were co-cultured for 24 hours, Th17 ratio in uncontrolled group and acute exacerbation of asthma was significantly decreased (P<0.05), and Treg ratio was significantly increased (P<0.05). The ratios of Th17 and Treg in partly controlled group were not different. Th17 ratio in controlled group was slightly decreased than before (P<0.05), but Treg ratio was not different.ConclusionsIn vitro, artemisinin has a certain regulation function on bronchial asthma of peripheral blood Th17/Treg, especially in refractory asthma. Artemisinin can improve in some degree the imbalance of TH17/Treg in CD4+ lymphocytes in patients with refractory asthma.
