CAJ | 학술논문

研究3类表面活性剂：非离子表面活性剂(PEG20000,Span80和吐温80)、阴离子表面活性剂(硬脂酸钙)和两性离子表面活性剂(甜菜碱和大豆磷脂)对瘤胃微生物体外发酵和纤维素降解酶活力的影响。结果表明,添加0.1％的 PEG20000、甜菜碱或大豆磷脂后,木聚糖酶活力分别提高35.4％(P ＜0.05),34.6％(P ＜0.05),26.8％(P ＜0.05)；Span80、甜菜碱、大豆磷脂和硬脂酸钙使内切β-1,4-葡聚糖酶活力分别提高27.1％(P ＜0.05),78.9％(P ＜0.05),90.3％(P ＜0.05),37.4％(P ＜0.05)。甜菜碱和大豆磷脂使小麦秸秆干物质降解率提高29.6％(P ＜0.05)和39.9％(P ＜0.05),中性洗涤纤维降解率分别增加54.6％(P ＜0.05)和91.8％(P ＜0.05)。当大豆磷脂和PEG20000添加水平增加到0.2％时,木聚糖酶增加48.9％(P ＜0.05)和46.0％(P ＜0.05)。添加0.2％的PEG20000和大豆磷脂后干物质降解率提高29.9％(P ＜0.05)和30.6％(P ＜0.05),中性洗涤纤维降解率提高42.3％(P ＜0.05)和69.1％(P ＜0.05)。在体外条件下,大豆磷脂、PEG20000和甜菜碱通过提高瘤胃微生物木聚糖酶、内切β-1,4-葡聚糖酶活力增加小麦秸秆干物质和中性洗涤纤维降解率。综合来看,表面活性剂对瘤胃发酵影响程度的顺序依次为：大豆磷脂＞PEG20000＞甜菜碱＞吐温80＞硬脂酸钙＞Span80。
연구3류표면활성제：비리자표면활성제(PEG20000,Span80화토온80)、음리자표면활성제(경지산개)화량성리자표면활성제(첨채감화대두린지)대류위미생물체외발효화섬유소강해매활력적영향。결과표명,첨가0.1％적 PEG20000、첨채감혹대두린지후,목취당매활력분별제고35.4％(P ＜0.05),34.6％(P ＜0.05),26.8％(P ＜0.05)；Span80、첨채감、대두린지화경지산개사내절β-1,4-포취당매활력분별제고27.1％(P ＜0.05),78.9％(P ＜0.05),90.3％(P ＜0.05),37.4％(P ＜0.05)。첨채감화대두린지사소맥갈간간물질강해솔제고29.6％(P ＜0.05)화39.9％(P ＜0.05),중성세조섬유강해솔분별증가54.6％(P ＜0.05)화91.8％(P ＜0.05)。당대두린지화PEG20000첨가수평증가도0.2％시,목취당매증가48.9％(P ＜0.05)화46.0％(P ＜0.05)。첨가0.2％적PEG20000화대두린지후간물질강해솔제고29.9％(P ＜0.05)화30.6％(P ＜0.05),중성세조섬유강해솔제고42.3％(P ＜0.05)화69.1％(P ＜0.05)。재체외조건하,대두린지、PEG20000화첨채감통과제고류위미생물목취당매、내절β-1,4-포취당매활력증가소맥갈간간물질화중성세조섬유강해솔。종합래간,표면활성제대류위발효영향정도적순서의차위：대두린지＞PEG20000＞첨채감＞토온80＞경지산개＞Span80。
Effects of different inclusion levels (0.1% or 0.2% in final concentration)of anionic surfactant (calcium stearate),zwitterionic surfactants (soy lecithin and betaine)and non-ionic surfactants (Tween80, PEG20000 and Span80)on fiber degrading enzyme activities of ruminal microorganisms and substrate de-gradability after 24h in vitro fermentation were investigated.The activity of xylanase were increased by 35.4%,34.6% and 26.8%(P <0.05 )after increase of 0.1% PEG20000,betaine and soy lecithin,respec-tively.In addition,after addition of 0.1% Span80,betaine,soy lecithin and calcium stearate,endo-β-1,4- glu-canase activity were improved by 27.1%,78.9%,90.3% and 37.4% (P <0.05),respectively.As a result, DM degradability of wheat straw increased by 29.6% and 39.9% (P <0.05 )and NDF degradability in-creased by 54.6% and 91.8% (P <0.05)after increase of betaine and soy lecithin.When soy lecithin and PEG20000 supplement level were increased by 0.2%,the xylanase increased by 48.9% and 46.0% (P <0.05),and the DM degradability of wheat straw increased by 29.9% and 30.6% (P <0.05),and NDF de-gradability increased by 42.3% and 69.1% (P <0.05).The results suggest that the action mechanisms of soybean lecithin ,PEG 2 0 0 0 0 and betaine improved xylanase and endo-β-1 ,4-glucanase activity ,and thus improving the degradation of DM and NDF of wheat straw.In general,the order of these surfactants in reg-ulating ruminal fermentation is as follows:soy lecithin>PEG20000>betaine>Tween 80 >calcium stea-rate>Span80.