CAJ | 학술논문

为了研究不同营养配比对新疆红富士苹果果实糖分积累相关酶活性的影响,以新疆霍城县的6 a生红富士苹果树为研究对象,采用氮、磷、钾三因素的二次回归正交施肥试验设计,在苹果生育期内进行了田间施肥试验,对苹果的淀粉酶活性、蔗糖合成酶活性、蔗糖磷酸合成酶活性进行了测定,分析了不同施肥处理与果实糖分积累相关酶活性的关系。结果表明,果实发育过程中淀粉酶活性总体呈下降的趋势,其中在幼果期为最大值8.38 mg/g,在着色期达到最小值为0.04 mg/g；蔗糖合成酶活性总体呈急剧上升趋势,其中在幼果期未检出,而在成熟期达到了最大值为61.78 mg/(g?L)；蔗糖磷酸合成酶活性总体呈缓慢上升趋势,但上升幅度不大,其中在幼果期与蔗糖合成酶一样未检出,而在成熟期达到了最大值为3.66 mg/(g?L)。通过回归分析得以成熟红富士苹果蔗糖合成酶活性值为经济目标时各个因素的最佳组合为：蔗糖合成酶活性为184.445 mg/(g?L),施氮量1.3090 kg/株。各因素对蔗糖合成酶活性影响的顺序：钾＞磷＞氮。氮与磷、氮与钾均呈负交互作用,磷与钾呈正交互作用。
위료연구불동영양배비대신강홍부사평과과실당분적루상관매활성적영향,이신강곽성현적6 a생홍부사평과수위연구대상,채용담、린、갑삼인소적이차회귀정교시비시험설계,재평과생육기내진행료전간시비시험,대평과적정분매활성、자당합성매활성、자당린산합성매활성진행료측정,분석료불동시비처리여과실당분적루상관매활성적관계。결과표명,과실발육과정중정분매활성총체정하강적추세,기중재유과기위최대치8.38 mg/g,재착색기체도최소치위0.04 mg/g；자당합성매활성총체정급극상승추세,기중재유과기미검출,이재성숙기체도료최대치위61.78 mg/(g?L)；자당린산합성매활성총체정완만상승추세,단상승폭도불대,기중재유과기여자당합성매일양미검출,이재성숙기체도료최대치위3.66 mg/(g?L)。통과회귀분석득이성숙홍부사평과자당합성매활성치위경제목표시각개인소적최가조합위：자당합성매활성위184.445 mg/(g?L),시담량1.3090 kg/주。각인소대자당합성매활성영향적순서：갑＞린＞담。담여린、담여갑균정부교호작용,린여갑정정교호작용。
The experiment was carried out to study the effects of different fertilization ratio of N,P and K on related enzyme activities of sugar accumulation in Huocheng county,Xinjiang by using twice regression orthogonal design for fertilization test,the field fertilization experiment had been done in apple growth pe-riod,amylase activities,saccharose synzyme activities,saccharose phosphoic acid synzyme activities were determened,and the relations between different fertilization treatments and related synzyme activities of fruit sugar accumulation were analyzed.The results showed that activity of amylase in the process of fruit development overall were downward trend.In young fruit period,the maltose maximum was 8.38 mg/g, and in the color phase,the maltose minimum was 0.04 mg/g.The activity of sucrose synthase showed a trend of sharp rise in general,it was not checked out in young fruit period;during the ripening period,it reached the maximum value of 61.78 mg/(g?L);and in coloring period it was the turning point of the sharp increase.The activity of sucrose phosphate synthase was slowly rising trend overall,and the increase was not big,in young fruit period like sucrose synthetase was not checked out,during the ripening,it reached the maximum value of 3 .6 6 mg/(g?L ).Through model analysis ,the economic goals of sucrose activities synthase activity of the matured Red Fuji apple,the best combination of various factors are as fol-lows:Sucrose synthase was 184.445 mg/(g?L),and N application rate of 1.3090 kg/plant.The order of va-rious factors affecting sucrose Synzyme activities are as follows:K>P>N.N and P,N and K were negative interaction;P and K were positively interaction.