华南理工大学学报（自然科学版）
華南理工大學學報（自然科學版）
화남리공대학학보（자연과학판）
JOURNAL OF SOUTH CHINA UNIVERSITY OF TECHNOLOGY(NATURAL SCIENCE EDITION)
2013年
12期
115-119,124
,共6页
组成型毕赤酵母%木聚糖酶%发酵动力学%流加策略
組成型畢赤酵母%木聚糖酶%髮酵動力學%流加策略
조성형필적효모%목취당매%발효동역학%류가책략
constitutive Pichia pastoris%xylanase%fermentation kinetics%fed-batch strategy
为优化发酵工艺，通过单罐恒化培养对组成型毕赤酵母X-33产木聚糖酶XYL1的动力学进行了研究，并探讨了比生长速率与菌体生产率和产物生产率的关系．基于动力学研究，在50 L发酵罐中采用分段控制工艺表达XYL1．结果表明：比生长速率与葡萄糖浓度的关系基本符合Monod方程，最大比生长速率为0．35 h-1，饱和常数为0．38 g/L；当比生长速率介于0.024～0．212之间时，XYL1生成动力学模型可用一元二次方程描述；比底物消耗速率与比生长速率呈线性关系；最佳稀释速率为0．316 h-1，实验预测的最大菌体生产率为7.509 g/（L·h）；当产物生产率最大时，比生长速率达0．156 h-1；最终发酵菌湿重达（415±12）g/L，酶活达（2788±85）IU/mL，实现了XYL1的高效表达．
為優化髮酵工藝，通過單罐恆化培養對組成型畢赤酵母X-33產木聚糖酶XYL1的動力學進行瞭研究，併探討瞭比生長速率與菌體生產率和產物生產率的關繫．基于動力學研究，在50 L髮酵罐中採用分段控製工藝錶達XYL1．結果錶明：比生長速率與葡萄糖濃度的關繫基本符閤Monod方程，最大比生長速率為0．35 h-1，飽和常數為0．38 g/L；噹比生長速率介于0.024～0．212之間時，XYL1生成動力學模型可用一元二次方程描述；比底物消耗速率與比生長速率呈線性關繫；最佳稀釋速率為0．316 h-1，實驗預測的最大菌體生產率為7.509 g/（L·h）；噹產物生產率最大時，比生長速率達0．156 h-1；最終髮酵菌濕重達（415±12）g/L，酶活達（2788±85）IU/mL，實現瞭XYL1的高效錶達．
위우화발효공예，통과단관항화배양대조성형필적효모X-33산목취당매XYL1적동역학진행료연구，병탐토료비생장속솔여균체생산솔화산물생산솔적관계．기우동역학연구，재50 L발효관중채용분단공제공예표체XYL1．결과표명：비생장속솔여포도당농도적관계기본부합Monod방정，최대비생장속솔위0．35 h-1，포화상수위0．38 g/L；당비생장속솔개우0.024～0．212지간시，XYL1생성동역학모형가용일원이차방정묘술；비저물소모속솔여비생장속솔정선성관계；최가희석속솔위0．316 h-1，실험예측적최대균체생산솔위7.509 g/（L·h）；당산물생산솔최대시，비생장속솔체0．156 h-1；최종발효균습중체（415±12）g/L，매활체（2788±85）IU/mL，실현료XYL1적고효표체．
In order to optimize the fermentation process of constitutive Pichia pastoris X-33 for producing xylanase 1 (XYL1 ),the fermentation kinetics during the single-tank chemostat cultivation was investigated,and the relation-ship between the specific cell growth rate and the cell productivity as well as the product yield was discussed. Then,based on the fermentation kinetics,batch control scheme was adopted to express XYL1 in a 50-L fermenta-tion tank.The results indicate that (1 )the relationship between the specific cell growth rate and the glucose con-centration basically accords with the Monod equation,with a maximum specific growth rate value of 0.35 h-1 and a saturation coefficient value of 0.38 g/L;(2)when the specific cell growth rate ranges from 0.024 to 0.212,the ki-netic model of XYL1 formation can be described using a quadratic equation with one unknown;(3 )the specific substrate consumption rate is linear to the specific cell growth rate;(4)the optimal dilution rate is 0.316 h-1 and the maximum volumetric cell productivity is 7.509g/(L·h);(5)the specific cell growth rate achieves 0.156h-1 when the product yield reaches its maximum;and (6)after fermentation,the wet weight of cells and the xylanase activity achieve (415 ±12)g/L and (2788 ±85)IU/mL,respectively,which means that XYL1 is successfully ex-pressed with high efficiency.
