CAJ | 학술논문

采用石英晶体微天平检测纤维素酶在麦草碱木质素薄膜上的吸附量以及吸附构型的变化，并研究了pH值对纤维素酶吸附的影响．研究发现：pH值在4．8～6．0范围内时，纤维素酶与麦草碱木质素之间存在静电斥力，而且pH值越高，纤维素酶与麦草碱木质素之间的静电斥力越大，纤维素酶在麦草碱木质素薄膜上的吸附越不牢固，形成的吸附膜越疏松，相应的吸附量也越少；同时，较高的pH值可以促使更多的纤维素酶从麦草碱木质素薄膜上脱附下来，提高纤维素酶的回收利用率．
채용석영정체미천평검측섬유소매재맥초감목질소박막상적흡부량이급흡부구형적변화，병연구료pH치대섬유소매흡부적영향．연구발현：pH치재4．8～6．0범위내시，섬유소매여맥초감목질소지간존재정전척력，이차pH치월고，섬유소매여맥초감목질소지간적정전척력월대，섬유소매재맥초감목질소박막상적흡부월불뢰고，형성적흡부막월소송，상응적흡부량야월소；동시，교고적pH치가이촉사경다적섬유소매종맥초감목질소박막상탈부하래，제고섬유소매적회수이용솔．
Quartz crystal microbalance was used to detect the adsorption capacity and configuration variation of cel-lulase on wheat straw alkaline lignin film,and the effect of pH value on the adsorption was investigated.The results indicate that (1 )there exists electrostatic repulsion between cellulase and wheat straw alkaline lignin when the pH value ranges from 4.8 to 6.0;(2)the electrostatic repulsion becomes stronger and the cellulase adsorption on the lignin film becomes more unfirm at a higher pH value,thus loosening the adsorbed film and decreasing the adsorp-tion capacity;and (3 )high pH value benefits the desorption of cellulase from wheat straw alkaline lignin film and its recovery.