CAJ | 학술논문

实时荧光定量PCR是在定性PCR技术基础上发展起来的核酸定量技术，它具有特异性强、PCR污染少、自动化程度高等特点。作者就实时荧光定量PCR技术目前在细菌、病毒、环境微生物等病原体领域的快速检测和诊断应用进行了概述。
실시형광정량PCR시재정성PCR기술기출상발전기래적핵산정량기술，타구유특이성강、PCR오염소、자동화정도고등특점。작자취실시형광정량PCR기술목전재세균、병독、배경미생물등병원체영역적쾌속검측화진단응용진행료개술。
Real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) was a technique of nucleic acid quantification which is developed on the basis of qualitative PCR technique. It has characteristic of strong specificity, less PCR contamination, and high degree automation. In this paper, we made an outline about present real-time PCR technology for rapid detection and diagnostic applications in bacteria, viruses, and other pathogens in the field of environmental microbiology .