中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
23期
10806-10808
,共3页
呼吸道合胞病毒%龙胆紫%空斑试验%羟甲基纤维素
呼吸道閤胞病毒%龍膽紫%空斑試驗%羥甲基纖維素
호흡도합포병독%룡담자%공반시험%간갑기섬유소
Respiratory syncytial viruses%Gentian violet%Plaque forming assay%Carboxymethylcellulose
目的：探讨甲基纤维素-结晶紫空斑法在呼吸道合胞病毒滴度检测中的应用。方法 Hep-2细胞在12孔细胞培养板中达到100%融合，感染呼吸道合胞病毒，用甲基纤维素代替琼脂糖培养5 d，用结晶紫代替中性红作为染色剂进行染色，最后计算空斑。结果呼吸道合胞病毒在甲基纤维素-结晶紫空斑法下形成的空斑清晰可见，大小合适，易于计算。结论甲基纤维素-结晶紫空斑法为呼吸道合胞病毒滴度的检测提供一种新的方法。
目的：探討甲基纖維素-結晶紫空斑法在呼吸道閤胞病毒滴度檢測中的應用。方法 Hep-2細胞在12孔細胞培養闆中達到100%融閤，感染呼吸道閤胞病毒，用甲基纖維素代替瓊脂糖培養5 d，用結晶紫代替中性紅作為染色劑進行染色，最後計算空斑。結果呼吸道閤胞病毒在甲基纖維素-結晶紫空斑法下形成的空斑清晰可見，大小閤適，易于計算。結論甲基纖維素-結晶紫空斑法為呼吸道閤胞病毒滴度的檢測提供一種新的方法。
목적：탐토갑기섬유소-결정자공반법재호흡도합포병독적도검측중적응용。방법 Hep-2세포재12공세포배양판중체도100%융합，감염호흡도합포병독，용갑기섬유소대체경지당배양5 d，용결정자대체중성홍작위염색제진행염색，최후계산공반。결과호흡도합포병독재갑기섬유소-결정자공반법하형성적공반청석가견，대소합괄，역우계산。결론갑기섬유소-결정자공반법위호흡도합포병독적도적검측제공일충신적방법。
Objective To evaluate application of carboxymethylcellulose-crystal violet plaque forming assay to determine the titer of respiratory syncytial virus(RSV). Methods To perform a plaque forming assay, RSV was inoculated onto Hep-2 cell monolayers when achieving 100%fusion, a single layer nutrient carboxymethylcellulose was added to cover cells and cultured for 5 days, crystal violet was used instead of neutral red to stain cells for counting virus plaque. Results The plaques by application of carboxymethylcellulose-crystal violet forming assay were revealed after 5 days on Hep-2 cell monolayers, the plaques of RSV were round in shape, 1.0 mm in diameter. Conclusion The carboxymethylcellulose-crystal violet forming assay could be a new method to determine the titer of RSV.
