中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
23期
10780-10783
,共4页
Akt%芬太尼%疼痛,手术后%痛觉过敏
Akt%芬太尼%疼痛,手術後%痛覺過敏
Akt%분태니%동통,수술후%통각과민
Akt%Remifentanil%Incisional pain%Hyperalgesia
目的观察造模前后鞘内注射Akt抑制剂Ⅳ对切口痛瑞芬太尼痛觉过敏小鼠痛行为学的影响。方法 C57BL/6小鼠40只，随机分为5组：切口痛＋DMSO组（I组，n＝8）、瑞芬太尼＋DMSO组（R 组，n＝8）造模前给予 Akt 抑制剂Ⅳ0.08μg/10μl 组（A1组，n＝8）、造模后6 h 给予 Akt 抑制剂Ⅳ0.08μg/10μl组（A2组，n＝8）、造模后1 d给予Akt抑制剂Ⅳ0.08μg/10μl组（A3组，n＝8）。所有分组均在右侧足做切口痛，R组和各A组同时皮下泵注瑞芬太尼（0.04 mg/kg）30 min。R组和各A组分别在术前30 min、术后6 h、1 d鞘内给予10%二甲基亚砜（DMSO）和Akt抑制剂Ⅳ10μl。于术前1 d及术后6 h、1、2、3、5、7 d检测各组小鼠右侧后足热缩足潜伏期（paw withdrawal thermal latency，PWTL）及机械缩足阈值（paw mechanical withdrawal threshold，PMWT）。结果与I组和基础值比较，R组术后各时间除术后7 d外PWMT和PWTL均降低（P＜0.05）；与R组相比，A1、A2组术后6 h、1 d、2 d及A3组术后1 d、2 d的PWMT和PWTL明显升高（P＜0.05）；A1、A2、A3组的各时间点的PWMT和PWTL有统计学差异（P＜0.05）。结论 Akt参与瑞芬太尼诱导的痛觉过敏，预先和造模后鞘内注射Akt抑制剂Ⅳ都能够有效缓解瑞芬太尼诱发的痛觉过敏。
目的觀察造模前後鞘內註射Akt抑製劑Ⅳ對切口痛瑞芬太尼痛覺過敏小鼠痛行為學的影響。方法 C57BL/6小鼠40隻，隨機分為5組：切口痛＋DMSO組（I組，n＝8）、瑞芬太尼＋DMSO組（R 組，n＝8）造模前給予 Akt 抑製劑Ⅳ0.08μg/10μl 組（A1組，n＝8）、造模後6 h 給予 Akt 抑製劑Ⅳ0.08μg/10μl組（A2組，n＝8）、造模後1 d給予Akt抑製劑Ⅳ0.08μg/10μl組（A3組，n＝8）。所有分組均在右側足做切口痛，R組和各A組同時皮下泵註瑞芬太尼（0.04 mg/kg）30 min。R組和各A組分彆在術前30 min、術後6 h、1 d鞘內給予10%二甲基亞砜（DMSO）和Akt抑製劑Ⅳ10μl。于術前1 d及術後6 h、1、2、3、5、7 d檢測各組小鼠右側後足熱縮足潛伏期（paw withdrawal thermal latency，PWTL）及機械縮足閾值（paw mechanical withdrawal threshold，PMWT）。結果與I組和基礎值比較，R組術後各時間除術後7 d外PWMT和PWTL均降低（P＜0.05）；與R組相比，A1、A2組術後6 h、1 d、2 d及A3組術後1 d、2 d的PWMT和PWTL明顯升高（P＜0.05）；A1、A2、A3組的各時間點的PWMT和PWTL有統計學差異（P＜0.05）。結論 Akt參與瑞芬太尼誘導的痛覺過敏，預先和造模後鞘內註射Akt抑製劑Ⅳ都能夠有效緩解瑞芬太尼誘髮的痛覺過敏。
목적관찰조모전후초내주사Akt억제제Ⅳ대절구통서분태니통각과민소서통행위학적영향。방법 C57BL/6소서40지，수궤분위5조：절구통＋DMSO조（I조，n＝8）、서분태니＋DMSO조（R 조，n＝8）조모전급여 Akt 억제제Ⅳ0.08μg/10μl 조（A1조，n＝8）、조모후6 h 급여 Akt 억제제Ⅳ0.08μg/10μl조（A2조，n＝8）、조모후1 d급여Akt억제제Ⅳ0.08μg/10μl조（A3조，n＝8）。소유분조균재우측족주절구통，R조화각A조동시피하빙주서분태니（0.04 mg/kg）30 min。R조화각A조분별재술전30 min、술후6 h、1 d초내급여10%이갑기아풍（DMSO）화Akt억제제Ⅳ10μl。우술전1 d급술후6 h、1、2、3、5、7 d검측각조소서우측후족열축족잠복기（paw withdrawal thermal latency，PWTL）급궤계축족역치（paw mechanical withdrawal threshold，PMWT）。결과여I조화기출치비교，R조술후각시간제술후7 d외PWMT화PWTL균강저（P＜0.05）；여R조상비，A1、A2조술후6 h、1 d、2 d급A3조술후1 d、2 d적PWMT화PWTL명현승고（P＜0.05）；A1、A2、A3조적각시간점적PWMT화PWTL유통계학차이（P＜0.05）。결론 Akt삼여서분태니유도적통각과민，예선화조모후초내주사Akt억제제Ⅳ도능구유효완해서분태니유발적통각과민。
Objective To investigate the effects of intrathecal injection of Akt inhibitor Ⅳ on the hyperalgesia induced by remifentanil in a incision pain model. Methods Forty C57BL/6 male mice were randomly divided into 5 groups (n=8): incision pain+DMSO group (group I), incision pain+remifentanil+DMSO group (group R), before modeling Akt inhibitor Ⅳ 0.08μg/10μl group (group A1), 6h after modeling Akt inhibitor Ⅳ 0.08μg/10μl group (group A2), 1d after modeling Akt inhibitor IV 0.08μg/10μl group (group A3). All groups were made model of incisional pain in the right paw, in group R and group A1, A2, A3, remifentanil (0.04 mg/kg) were infused subcutaneously 30 min at the moment of surgery. All groups were respectively intrathecal injected 10μl DMSO (10%) and the corresponding concentration of the Akt inhibitor Ⅳ 10 μl. Each mouse received tests of the paw mechanical withdrawal threshold (PMWT) and the paw withdrawal thermal latency (PWTL) at the times of 24 h before and 6 h, 1 d, 2 d, 3 d, 5 d, 7 d after surgery. Results Compared with group I and the baseline value, PWMT and PWTL were significantly decreased after surgery except 7 d in group R (P<0.05);Compared with group R, PWMT and PWTL were significantly increased after 6 h, 1 d, 2 d of surgery in groups A1, A2 and in group A3 (1 d, 2 d) (P<0.05); While there was statistical difference among group A1, A2, A3 (P>0.05). Conclusion Intrathecal injection of Akt inhibitor Ⅳ can effectively relieve the hyperalgesia induced by remifentanil on before and after modeling.
