CAJ | 학술논문

目的：观察过氧化物酶体增殖物激活受体-γ（peroxisome proliferator activated receptor-γ， PPAR-γ）的配体l5-脱氧-△12,14-前列腺素J2（15-deoxy-△12,14-prostagliandxin J2，15d-PGJ2）对兔耳增生性瘢痕CD34及转化生长因子-β1（transforming growth factor-β1，TGF-β1）的影响，探讨15d-PGJ2防治增生性瘢痕的机制和可行性。方法选取新西兰大白兔9只，在兔耳腹侧面制作2 cm×2 cm全层皮肤缺损创面，每耳2个，共计36个，建立兔耳增生性瘢痕动物模型，随机分为实验组和对照组，分别用15d-PGJ2及生理盐水行瘢痕内注射，1次/d，连续注射7 d。停止注射后第3、6、17天取材。应用免疫组织化学方法检测CD34和TGF-β1的表达。结果兔耳创面愈合后均出现不同程度类似人增生性瘢痕组织块，与对照组比较，实验组注射15d-PGJ2后瘢痕逐渐变平变软，颜色变浅，在各时间点CD34及TGF-β1的表达均较对照组低，差异有统计学意义（P＜0.05）。结论 PPAR-γ的配体15d-PGJ2可减少瘢痕内CD34、TGF-β1的含量，引起瘢痕萎缩，有一定的防治瘢痕增生的作用，为其治疗增生性瘢痕的临床应用提供了一定的理论依据。
목적：관찰과양화물매체증식물격활수체-γ（peroxisome proliferator activated receptor-γ， PPAR-γ）적배체l5-탈양-△12,14-전렬선소J2（15-deoxy-△12,14-prostagliandxin J2，15d-PGJ2）대토이증생성반흔CD34급전화생장인자-β1（transforming growth factor-β1，TGF-β1）적영향，탐토15d-PGJ2방치증생성반흔적궤제화가행성。방법선취신서란대백토9지，재토이복측면제작2 cm×2 cm전층피부결손창면，매이2개，공계36개，건립토이증생성반흔동물모형，수궤분위실험조화대조조，분별용15d-PGJ2급생리염수행반흔내주사，1차/d，련속주사7 d。정지주사후제3、6、17천취재。응용면역조직화학방법검측CD34화TGF-β1적표체。결과토이창면유합후균출현불동정도유사인증생성반흔조직괴，여대조조비교，실험조주사15d-PGJ2후반흔축점변평변연，안색변천，재각시간점CD34급TGF-β1적표체균교대조조저，차이유통계학의의（P＜0.05）。결론 PPAR-γ적배체15d-PGJ2가감소반흔내CD34、TGF-β1적함량，인기반흔위축，유일정적방치반흔증생적작용，위기치료증생성반흔적림상응용제공료일정적이론의거。
Objective To investigate the effect of 15d-PGJ2 on the expression of CD34 and TGF-β1 on hypertrophic scar in rabbit ear and the possibility of hypertrophic scar treated by 15d-PGJ2. Methods 9 New Zealand white rabbits were used to establish a hypertrophic scar model on the rabbit ear.The wounds were established as follows:2 cm×2 cm wounds with total skin loses on the ventral side, 2 wounds for each ear, totally 36 wounds. The wounds were randomly divided into the 15d-PGJ2 treatment group and normal saline control group. 10μl 15d-PGJ2 or normal saline was injected into the ear scar once a day for 7 days. At 3, 6 and 17 days after the injection, the expression of CD34 and TGF-β1 was detected by immunohistochemical method. Results Excessive dermal scars on rabbit ear that were similar to human hypertrophic scar appeared in the two groups. Compared with the NS-treated scar group, the 15d-PGJ2-treated scars appeared to be smaller, softer, flatter and lighter in color. The expression of CD34 and TGF-β1 in the 15d-PGJ2 group was significantly decreased as compared with that in the control group at different time points(P<0.05). Conclusion 15d-PGJ2, the ligand of PPAR-γ, can reduce the expression of CD34 and TGF-β1 of hypertrophic scar in the rabbit ear and plays an important role in the prevention and treatment of hypertrophic scar.