中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
23期
10762-10765
,共4页
TSA%胰腺癌细胞株%MICA/B
TSA%胰腺癌細胞株%MICA/B
TSA%이선암세포주%MICA/B
TSA%Pancreatic cancer cell lines%MICA/B
目的：研究细胞因子诱导的杀伤（CIK）细胞对胰腺癌细胞株的杀伤效果；探索曲古抑菌素A（TSA）增强其杀伤效果的机制。方法将胰腺癌细胞株分为2组。一组经100 mmol/L；200 mmol/L浓度的TSA处理24 h，另外一组为空白对照。分别使用流式细胞仪测定两组胰腺癌细胞株的MICA/B表达及使用乳酸脱氢酶法测定CIK细胞对两组胰腺癌细胞株的杀伤率。双变量相关性分析胰腺癌细胞株MICA/B的表达与CIK细胞对胰腺癌细胞株杀伤率的关系。结果经TSA处理24 h后的胰腺癌细胞株，其MICA/B表达相较对照组有明显的升高（P＝0.000），有统计学差异；在不同靶效比的情况下，CIK 细胞对经 TSA处理24 h后的胰腺癌细胞株的杀伤率有显著的提高（P＝0.000）；而相关性分析显示，CIK细胞对胰腺癌细胞株杀伤率的升高与胰腺癌细胞株MICA/B的表达呈正相关（P＜0.01）。结论 TSA可显著调高胰腺癌细胞株的MICA/B表达（P＜0.01），从而增强CIK细胞对胰腺癌细胞株的杀伤。
目的：研究細胞因子誘導的殺傷（CIK）細胞對胰腺癌細胞株的殺傷效果；探索麯古抑菌素A（TSA）增彊其殺傷效果的機製。方法將胰腺癌細胞株分為2組。一組經100 mmol/L；200 mmol/L濃度的TSA處理24 h，另外一組為空白對照。分彆使用流式細胞儀測定兩組胰腺癌細胞株的MICA/B錶達及使用乳痠脫氫酶法測定CIK細胞對兩組胰腺癌細胞株的殺傷率。雙變量相關性分析胰腺癌細胞株MICA/B的錶達與CIK細胞對胰腺癌細胞株殺傷率的關繫。結果經TSA處理24 h後的胰腺癌細胞株，其MICA/B錶達相較對照組有明顯的升高（P＝0.000），有統計學差異；在不同靶效比的情況下，CIK 細胞對經 TSA處理24 h後的胰腺癌細胞株的殺傷率有顯著的提高（P＝0.000）；而相關性分析顯示，CIK細胞對胰腺癌細胞株殺傷率的升高與胰腺癌細胞株MICA/B的錶達呈正相關（P＜0.01）。結論 TSA可顯著調高胰腺癌細胞株的MICA/B錶達（P＜0.01），從而增彊CIK細胞對胰腺癌細胞株的殺傷。
목적：연구세포인자유도적살상（CIK）세포대이선암세포주적살상효과；탐색곡고억균소A（TSA）증강기살상효과적궤제。방법장이선암세포주분위2조。일조경100 mmol/L；200 mmol/L농도적TSA처리24 h，령외일조위공백대조。분별사용류식세포의측정량조이선암세포주적MICA/B표체급사용유산탈경매법측정CIK세포대량조이선암세포주적살상솔。쌍변량상관성분석이선암세포주MICA/B적표체여CIK세포대이선암세포주살상솔적관계。결과경TSA처리24 h후적이선암세포주，기MICA/B표체상교대조조유명현적승고（P＝0.000），유통계학차이；재불동파효비적정황하，CIK 세포대경 TSA처리24 h후적이선암세포주적살상솔유현저적제고（P＝0.000）；이상관성분석현시，CIK세포대이선암세포주살상솔적승고여이선암세포주MICA/B적표체정정상관（P＜0.01）。결론 TSA가현저조고이선암세포주적MICA/B표체（P＜0.01），종이증강CIK세포대이선암세포주적살상。
Objective To study on pancreatic cancer cell lines CIK killing effect;explore TSA mechanisms to enhance its lethal effect. Methods Pancreatic cancer cell lines are divided into two groups. One group was the cell of 100 mmol/L; 200 mmol/L for 24 hours TSA concentration, the other group was the control group. Respectively, flow cytometry determined two group pancreatic cancer cell lines MICA/B expression; of lactate dehydrogenase methods measured the killing rates of two groups of CIK to pancreatic cancer cell lines. Bivariate correlation analysis of the relationship of the MICA/B expression in pancreatic cancer cell lines and the CIK killing rates. Results Using TSA for 24 hours, the MICA/B expression of pancreatic cancer cell lines increased significantly, which compared to the control group (P=0.000), with statistically significant differences. CIK killing rates of pancreatic cancer cell lines was significantly increased which was using TSA treatment for 24 hours (P=0.000);Bivariate correlation showed that, the relationship of CIK killing rates of pancreatic cancer cell lines and the MICA/B expression was positive correlation (P<0.01). Conclusion TSA can significantly increase the MICA/B expression of pancreatic cancer cell lines (P<0.01), thereby enhancing CIK killing rates of pancreatic cancer cell lines.
