中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
23期
10752-10757
,共6页
彭卫华%南丽红%赖文芳%贾铷%吴符火%徐曾涛
彭衛華%南麗紅%賴文芳%賈銣%吳符火%徐曾濤
팽위화%남려홍%뢰문방%가여%오부화%서증도
筋骨草属%肾小球肾炎,膜增生性%NF-κB%转化生长因子β1
觔骨草屬%腎小毬腎炎,膜增生性%NF-κB%轉化生長因子β1
근골초속%신소구신염,막증생성%NF-κB%전화생장인자β1
Ajuga%Glomerulonephritis,membranoproliferative%NF-kappa B%Transforming growth factor beta1
目的：探讨筋骨草总黄酮（TFA）治疗系膜增生性肾小球肾炎（MsPGN）大鼠后核转录因子-κB（NF-κB）及下游转化生长因子-β1（TGF-β1）的变化。方法采用改良慢性血清病MsPGN大鼠模型，于造模第5周末检测尿蛋白，将尿蛋白阳性者随机分为模型组、雷公藤多甙（TWP）组、不同剂量TFA组。于造模第6周开始给药，6周后，测定各组大鼠的24 h尿蛋白定量、血生化和肾组织病理变化，MaxVisionTM即用型快速免疫组化一步法检测大鼠肾组织中NF-κB p65的表达，双抗体夹心法检测血清中TGF-β1的变化。结果 TFA可显著降低尿蛋白，明显减小系膜区面积占毛细血管丛面积的百分比（P＜0.05或P＜0.01）；高、中剂量的TFA治疗效果与TWP相近（P＞0.05）。模型对照组大鼠肾组织NF-κB p65、血清TGF-β1的表达明显高于正常对照组[NF-κB p65：28.93±4.45 vs.4.51±0.96，P＜0.01；TGF-β1：（247.63±51.55）pg/ml vs.（127.52±27.84）pg/ml，P＜0.01]；干预性治疗后，TFA高、中剂量组、TWP组大鼠血清中NF-κB p65、TGF-β1的表达[NF-κB p65：10.35±2.81，15.21±4.71，11.70±3.97，P＜0.01；TGF-β1：（181.96±52.93） pg/ml，（204.33±38.30）pg/ml，（188.07±42.92）pg/ml，P＜0.05]较模型对照组明显降低。结论 TFA降低NF-κB并进而降低TGF-β1可能是治疗MsPGN大鼠的作用机制之一。
目的：探討觔骨草總黃酮（TFA）治療繫膜增生性腎小毬腎炎（MsPGN）大鼠後覈轉錄因子-κB（NF-κB）及下遊轉化生長因子-β1（TGF-β1）的變化。方法採用改良慢性血清病MsPGN大鼠模型，于造模第5週末檢測尿蛋白，將尿蛋白暘性者隨機分為模型組、雷公籐多甙（TWP）組、不同劑量TFA組。于造模第6週開始給藥，6週後，測定各組大鼠的24 h尿蛋白定量、血生化和腎組織病理變化，MaxVisionTM即用型快速免疫組化一步法檢測大鼠腎組織中NF-κB p65的錶達，雙抗體夾心法檢測血清中TGF-β1的變化。結果 TFA可顯著降低尿蛋白，明顯減小繫膜區麵積佔毛細血管叢麵積的百分比（P＜0.05或P＜0.01）；高、中劑量的TFA治療效果與TWP相近（P＞0.05）。模型對照組大鼠腎組織NF-κB p65、血清TGF-β1的錶達明顯高于正常對照組[NF-κB p65：28.93±4.45 vs.4.51±0.96，P＜0.01；TGF-β1：（247.63±51.55）pg/ml vs.（127.52±27.84）pg/ml，P＜0.01]；榦預性治療後，TFA高、中劑量組、TWP組大鼠血清中NF-κB p65、TGF-β1的錶達[NF-κB p65：10.35±2.81，15.21±4.71，11.70±3.97，P＜0.01；TGF-β1：（181.96±52.93） pg/ml，（204.33±38.30）pg/ml，（188.07±42.92）pg/ml，P＜0.05]較模型對照組明顯降低。結論 TFA降低NF-κB併進而降低TGF-β1可能是治療MsPGN大鼠的作用機製之一。
목적：탐토근골초총황동（TFA）치료계막증생성신소구신염（MsPGN）대서후핵전록인자-κB（NF-κB）급하유전화생장인자-β1（TGF-β1）적변화。방법채용개량만성혈청병MsPGN대서모형，우조모제5주말검측뇨단백，장뇨단백양성자수궤분위모형조、뢰공등다대（TWP）조、불동제량TFA조。우조모제6주개시급약，6주후，측정각조대서적24 h뇨단백정량、혈생화화신조직병리변화，MaxVisionTM즉용형쾌속면역조화일보법검측대서신조직중NF-κB p65적표체，쌍항체협심법검측혈청중TGF-β1적변화。결과 TFA가현저강저뇨단백，명현감소계막구면적점모세혈관총면적적백분비（P＜0.05혹P＜0.01）；고、중제량적TFA치료효과여TWP상근（P＞0.05）。모형대조조대서신조직NF-κB p65、혈청TGF-β1적표체명현고우정상대조조[NF-κB p65：28.93±4.45 vs.4.51±0.96，P＜0.01；TGF-β1：（247.63±51.55）pg/ml vs.（127.52±27.84）pg/ml，P＜0.01]；간예성치료후，TFA고、중제량조、TWP조대서혈청중NF-κB p65、TGF-β1적표체[NF-κB p65：10.35±2.81，15.21±4.71，11.70±3.97，P＜0.01；TGF-β1：（181.96±52.93） pg/ml，（204.33±38.30）pg/ml，（188.07±42.92）pg/ml，P＜0.05]교모형대조조명현강저。결론 TFA강저NF-κB병진이강저TGF-β1가능시치료MsPGN대서적작용궤제지일。
Objective To investigate the effects of total flavonoids of Ajuga (TFA) on nuclear transcriptional factor of kappa B (NF-κB) in mesangial proliferative glomerulonephritis (MsPGN) rats. Methods MsPGN rat was induced by the improved chronic serum sickness model after five weeks. the positive urinary protein rats were randomly divided into model group, triptergium wilfordii polyglycosidium (TWP) group and TFA group with different dosage, normal rats was considered as the control group. MsPGN rats were treated with drugs since the sixth week; succeeding six weeks later,all rats were detected 24 h urinary protein, blood biochemistry, NF-κB change in renal tissue by immunohistochemical method, serum transforming growth factor beta 1 (TGF-β1) was measured by double antibody sandwich ELISA immunoassay. Results Compared with model group, TFA can significantly reduce the urinary protein, decrease BUN, Scr, TG, TC, mitigate the morphological damage (P<0.05 or P<0.01);The effects of high-dosage (2.160 g/kg) TFA and middle-dosage (1.080 g/kg) TFA were as similar as those of TWP (P>0.05). The renal tissue NF-κB (Integral optical density, IOD) and serum TGF-β1 (pg/ml) in model control was significantly higher than that in normal control group, NF-κB p65 28.93±4.45 vs. 4.51±0.96, P<0.01; TGF-β1: (247.63±51.55)pg/ml vs. (127.52±27.84)pg/ml, P<0.01; With the intervention trerapy of high-dosage TFA, middle-dosage TFA and TWP, the expression of NF-κB in renal tissue and TGF-β1 in serum was significantly decreased compared with model control group, NF-κB p65: 10.35±2.81, 15.21±4.71,11.70±3.97, P<0.01; TGF-β1: (181.96±52.93)pg/ml, (204.33±38.30)pg/ml, (188.07±42.92)pg/ml, P<0.05. Conclusion Reducing the expression of NF-κB and successive TGF-β1 may be one of the mechanisms of total flavonoids of Ajuga treatment on MsPGN rats.
