CAJ | 학술논문

目的：探讨上皮性卵巢癌中癌相关成纤维细胞（CAFs）与临床病理特征间的相关性。方法应用免疫组化通用型二步法检测145例上皮性卵巢癌中平滑肌肌动蛋白（α-SMA）和基质金属蛋白酶-9（MMP-9）的表达，并分析两者的相关性及其临床意义。结果（1）正常卵巢组织除卵巢间质血管外，α-SMA不表达。在145例上皮性卵巢癌中，α-SMA阳性的CAFs细胞在癌组织中的分布有3种方式；且Ⅲ～Ⅳ期卵巢癌间质α-SMA阳性表达强度明显高于Ⅰ～Ⅱ期（P＝0.016）；有淋巴结转移的卵巢癌明显高于无淋巴结转移卵巢癌（P＝0.049）。（2）正常卵巢组织中MMP-9不表达；而上皮性癌中癌细胞MMP-9阳性率为95.2%（138/145）。MMP-9在Ⅲ～Ⅳ期组明显高于Ⅰ～Ⅱ期组（P＝0.028），有淋巴结转移组强阳性率高于无转移组（P＝0.033）。（3）α-SMA在上皮性卵巢癌间质强阳性表达与癌细胞 MMP-9的表达呈正相关（P＝0.027）。结论α-SMA 和 MMP-9两者的共同强阳性表达主要集中于Ⅲ～Ⅳ期癌患者和有淋巴结转移的患者中，CAFs的存在与数量增多将成为上皮性卵巢癌预后不良的一项独立指标。
목적：탐토상피성란소암중암상관성섬유세포（CAFs）여림상병리특정간적상관성。방법응용면역조화통용형이보법검측145례상피성란소암중평활기기동단백（α-SMA）화기질금속단백매-9（MMP-9）적표체，병분석량자적상관성급기림상의의。결과（1）정상란소조직제란소간질혈관외，α-SMA불표체。재145례상피성란소암중，α-SMA양성적CAFs세포재암조직중적분포유3충방식；차Ⅲ～Ⅳ기란소암간질α-SMA양성표체강도명현고우Ⅰ～Ⅱ기（P＝0.016）；유림파결전이적란소암명현고우무림파결전이란소암（P＝0.049）。（2）정상란소조직중MMP-9불표체；이상피성암중암세포MMP-9양성솔위95.2%（138/145）。MMP-9재Ⅲ～Ⅳ기조명현고우Ⅰ～Ⅱ기조（P＝0.028），유림파결전이조강양성솔고우무전이조（P＝0.033）。（3）α-SMA재상피성란소암간질강양성표체여암세포 MMP-9적표체정정상관（P＝0.027）。결론α-SMA 화 MMP-9량자적공동강양성표체주요집중우Ⅲ～Ⅳ기암환자화유림파결전이적환자중，CAFs적존재여수량증다장성위상피성란소암예후불량적일항독립지표。
Objective To investigate the relationship between the distribution of carcinoma-associated fibroblasts (CAFs) and their clinicopathological features as well as patient prognosis in epithelial ovarian cancer (EOC). Methods We used immunohistochemical technique to detect the expression of α-SMA and MMP-9 in 145 cases of EOC, and analyzed its relationship with clinicopathological features. Results The expression ofα-SMA in CAFs was upregulated as compared with normal tissue. Although the expression of α-SMA had no obvious relationship with age, tumor size and histological grade, it was associated with the clinical stage and lymph node metastases (P<0.05). The expression of MMP-9 in EOC cells was upregulated compared with normal tissue.The expression of MMP-9 was associated with the clinical stage and lymph node metastases (P<0.05), but had no obvious relationship with histological grade, tumor size and histological type (P>0.05). In addition, the expression ofα-SMA in CAFs was correlated with the expression of MMP-9 in cancer cells. Conclusion The CAFs in EOC seem to play an important role in the ovarian cancer progression and lymph node metastases. It may become potential prognostic predictors of EOC.