中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
23期
10611-10613
,共3页
何莉%任微%梁雪妮%刘蒙蒙%孙炳奇%孟冬娅
何莉%任微%樑雪妮%劉矇矇%孫炳奇%孟鼕婭
하리%임미%량설니%류몽몽%손병기%맹동아
分枝杆菌属%基因芯片%罗氏培养
分枝桿菌屬%基因芯片%囉氏培養
분지간균속%기인심편%라씨배양
Mycobacterium%Gene chip%Lowenstein-Jensen culture
目的：比较DNA-微阵列芯片法和改良罗氏法检测结核分枝杆菌和非结核分枝杆菌的一致性，探讨DNA-微阵列芯片法在临床上的应用价值。方法应用DNA-微阵列芯片法检测64例改良罗氏法阳性的分枝杆菌核酸，比较两种方法检测结果的一致率；两种方法检测结果不一致样本进行测序验证，以测序结果作为金标准，分析两种方法检测结果的正确率及不一致的可能原因。结果两种方法检测总一致率为93.8%，结核分枝杆菌检测一致率为87.5%，非结核分枝杆菌检测一致率为95.8%。DNA-微阵列芯片法和改良罗氏法共有4例检测结果不一致，与测序结果比对后，改良罗氏法区分结核和非结核的正确率为96.9%；DNA-微阵列芯片法正确率为93.8%，且能鉴定出7种常见分枝杆菌。结论 DNA-微阵列芯片法与作为传统金标准的改良罗氏法检测结核分枝杆菌和非结核分枝杆菌的一致性好、正确率高。操作简单、快速、灵敏、并且能鉴定出常见分枝杆菌，DNA-微阵列芯片法可作为临床分枝杆菌感染诊断的重要检测手段。
目的：比較DNA-微陣列芯片法和改良囉氏法檢測結覈分枝桿菌和非結覈分枝桿菌的一緻性，探討DNA-微陣列芯片法在臨床上的應用價值。方法應用DNA-微陣列芯片法檢測64例改良囉氏法暘性的分枝桿菌覈痠，比較兩種方法檢測結果的一緻率；兩種方法檢測結果不一緻樣本進行測序驗證，以測序結果作為金標準，分析兩種方法檢測結果的正確率及不一緻的可能原因。結果兩種方法檢測總一緻率為93.8%，結覈分枝桿菌檢測一緻率為87.5%，非結覈分枝桿菌檢測一緻率為95.8%。DNA-微陣列芯片法和改良囉氏法共有4例檢測結果不一緻，與測序結果比對後，改良囉氏法區分結覈和非結覈的正確率為96.9%；DNA-微陣列芯片法正確率為93.8%，且能鑒定齣7種常見分枝桿菌。結論 DNA-微陣列芯片法與作為傳統金標準的改良囉氏法檢測結覈分枝桿菌和非結覈分枝桿菌的一緻性好、正確率高。操作簡單、快速、靈敏、併且能鑒定齣常見分枝桿菌，DNA-微陣列芯片法可作為臨床分枝桿菌感染診斷的重要檢測手段。
목적：비교DNA-미진렬심편법화개량라씨법검측결핵분지간균화비결핵분지간균적일치성，탐토DNA-미진렬심편법재림상상적응용개치。방법응용DNA-미진렬심편법검측64례개량라씨법양성적분지간균핵산，비교량충방법검측결과적일치솔；량충방법검측결과불일치양본진행측서험증，이측서결과작위금표준，분석량충방법검측결과적정학솔급불일치적가능원인。결과량충방법검측총일치솔위93.8%，결핵분지간균검측일치솔위87.5%，비결핵분지간균검측일치솔위95.8%。DNA-미진렬심편법화개량라씨법공유4례검측결과불일치，여측서결과비대후，개량라씨법구분결핵화비결핵적정학솔위96.9%；DNA-미진렬심편법정학솔위93.8%，차능감정출7충상견분지간균。결론 DNA-미진렬심편법여작위전통금표준적개량라씨법검측결핵분지간균화비결핵분지간균적일치성호、정학솔고。조작간단、쾌속、령민、병차능감정출상견분지간균，DNA-미진렬심편법가작위림상분지간균감염진단적중요검측수단。
Objective To compare the consistency of mycobacterium tuberculosis and non-tuberculous mycobacteria detected by Gene chip and Lowenstein-Jensen, then explore the value Gene chip clinical application. Methods 64 cases of Lowenstein-Jensen positive mycobacterial nucleic acid were detected by Gene chip to compare and analyse the consistency rate of two results and the possible reasons of incongruity results which would be verified by sequencing which is believed as the gold standard. Results The total concordance rate total concordance rate of two detecting methods was 93.8%, mycobacterium tuberculosis concordance rate was 87.5%, non-tuberculous mycobacteria was 95.8%. Comparing 4 cases of incongruity with sequencing results, Lowenstein-Jensen precision rate was 96.9%and Gene chip which can identify seven kinds of common mycobacterium was 93.8%in distinguishing MTB and NTM. Conclusion Gene chip and Lowenstein-Jensen which is considered as traditional gold standard have good consistency and high accuracy, so Gene chip which is easily operation,rapid, sensitivity and identifying common mycobacterium can be regarded as an important method in clinical diagnosis of mycobacterial infection.
