CAJ | 학술논문

目的：筛选刺激机体产生抗体的铜绿假单胞菌优势抗原表位，分析铜绿假单胞菌抗原基因的定位。方法收集健康人和铜绿假单胞菌感染患者血清，应用噬菌体展示十二肽库进行1轮阴性选择淘选和3轮生物淘选，对阳性克隆单噬菌体进行测序，与PAO1基因组序列比对分析。结果30个阳性克隆单噬菌体，测序得到12种序列。与PAO1基因组序列比对得到673个基因，细胞定位为胞浆、内膜、周质、外膜、胞外和未知，所占比例分别为49%、22%、6%、6%、2%和15%。基因产物按功能分为26类，各部位的基因与细胞功能一致。结论推测胞外和外膜中“细胞壁/脂多糖/荚膜”类、“分泌因子”类和“运动及黏附”类抗原可用于疫苗研究。
목적：사선자격궤체산생항체적동록가단포균우세항원표위，분석동록가단포균항원기인적정위。방법수집건강인화동록가단포균감염환자혈청，응용서균체전시십이태고진행1륜음성선택도선화3륜생물도선，대양성극륭단서균체진행측서，여PAO1기인조서렬비대분석。결과30개양성극륭단서균체，측서득도12충서렬。여PAO1기인조서렬비대득도673개기인，세포정위위포장、내막、주질、외막、포외화미지，소점비례분별위49%、22%、6%、6%、2%화15%。기인산물안공능분위26류，각부위적기인여세포공능일치。결론추측포외화외막중“세포벽/지다당/협막”류、“분비인자”류화“운동급점부”류항원가용우역묘연구。
Objective To identify epitopes of Pseudomonas aeruginosa dominant antigens that can stimulate the body to produce antibodies and analyze the location of antigen genes. Methods The sera collected from controls and patients infected by Pseudomonas aeruginosa were used to screen the immunodominant of mimic epitopes of PA by a random 12-peptide phage display through one negative selection panning and three rounds biopanning. The positive phages clones were sequenced and then compared with the published sequence of strain PAO1. Results After four rounds of biopanning, a significant enrichment of phages were achieved. The inserts fron 30 positive phages clones were devided into 12 peptides based on different amino acids sequence. Through comparison with PAO1 genomic sequences, we got 673 genes, whose cellular localization were cytoplasm, cytoplasmic membrane, periplasm, outer membrane, extracellular and unknown, and the proportions were 49%, 22%, 6%, 6%, 2%and 15%respectively. Gene products could be classified into 26 categories, and the genes in each part of the cells were in accordance with its function. Conclusion We speculated that the antigens of"cell wall/LPS/capsule","secreted factors"and"motility and attachment"could be used for vaccine research.