中华临床医师杂志(电子版)
中華臨床醫師雜誌(電子版)
중화림상의사잡지(전자판)
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
23期
10422-10425
,共4页
汪建林%戴斌%杨西胜%高远%阮柏%申炜炜%李小磊%李海民
汪建林%戴斌%楊西勝%高遠%阮柏%申煒煒%李小磊%李海民
왕건림%대빈%양서성%고원%원백%신위위%리소뢰%리해민
肝肿瘤%miR-200a%分子诊断技术
肝腫瘤%miR-200a%分子診斷技術
간종류%miR-200a%분자진단기술
Liver neoplasms%miR-200a%Molecular diagnostic techniques
目的:探讨肝癌患者血清 miR-200a 表达及其临床病理特征之间的关系。方法运用实时定量PCR技术检测36例肝癌患者和21例健康人血清标本中miR-200a的表达情况,分析血清miR-200a的表达水平与肝癌患者临床病理参数之间的关系。血清中 AFP 值从临床资料中获取。结果36例肝癌患者miR-200a的表达水平为4.63±1.31,21例健康人对照为6.05±0.51,两者差异有统计学意义(P<0.05)。在36例肝癌患者中,血清miR-200a表达水平与肝癌分化程度、TNM分期及AFP有关(P<0.05),而与性别、年龄、抽烟、饮酒、HBV、肝硬化无关(P>0.05)。结论血清 miR-200a 表达水平可作为新的生物学指标对肝癌的诊断具有一定的价值。
目的:探討肝癌患者血清 miR-200a 錶達及其臨床病理特徵之間的關繫。方法運用實時定量PCR技術檢測36例肝癌患者和21例健康人血清標本中miR-200a的錶達情況,分析血清miR-200a的錶達水平與肝癌患者臨床病理參數之間的關繫。血清中 AFP 值從臨床資料中穫取。結果36例肝癌患者miR-200a的錶達水平為4.63±1.31,21例健康人對照為6.05±0.51,兩者差異有統計學意義(P<0.05)。在36例肝癌患者中,血清miR-200a錶達水平與肝癌分化程度、TNM分期及AFP有關(P<0.05),而與性彆、年齡、抽煙、飲酒、HBV、肝硬化無關(P>0.05)。結論血清 miR-200a 錶達水平可作為新的生物學指標對肝癌的診斷具有一定的價值。
목적:탐토간암환자혈청 miR-200a 표체급기림상병리특정지간적관계。방법운용실시정량PCR기술검측36례간암환자화21례건강인혈청표본중miR-200a적표체정황,분석혈청miR-200a적표체수평여간암환자림상병리삼수지간적관계。혈청중 AFP 치종림상자료중획취。결과36례간암환자miR-200a적표체수평위4.63±1.31,21례건강인대조위6.05±0.51,량자차이유통계학의의(P<0.05)。재36례간암환자중,혈청miR-200a표체수평여간암분화정도、TNM분기급AFP유관(P<0.05),이여성별、년령、추연、음주、HBV、간경화무관(P>0.05)。결론혈청 miR-200a 표체수평가작위신적생물학지표대간암적진단구유일정적개치。
Objective To investigate the expression level of miR-200a in serum of patients with hepatocellular carcinoma and its clinical significance. Methods Real time PCR was used to detect the expression of miR-200a in the serum of 36 patients with hepatocellular carcinoma and 21 normal controls, to analysis the relationship between the expression of serum miR-200a and clinical pathological parameters in patients with hepatocellular carcinoma. The AFP expression was got from clinical data. Results The expression level of miR-200a was 4.63 ±1.31 in 36 hepatocellular carcinoma patients and 6.05±0.51 in 21 normal control .Serum miR-200a expression level in 36 patients was significantly lower than that in normal control (P<0.05). Serum miR-200a expression was significantly associated with tumor differentiation, TNM and AFP (P<0.05). There were no correlation between serum miR-200a expression level and sex, age, smoked cigarettes, consumed alcohol, HBV, cirrhosis(P>0.05). Conclusion Our data confirms a role for serum miR-200a as a novel cancer biomarker in hepatocellular carcinoma.