中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
1期
112-114
,共3页
范宁建%冯世庆%刘举%崔猛%周先虎%吴秋丽
範寧建%馮世慶%劉舉%崔猛%週先虎%吳鞦麗
범저건%풍세경%류거%최맹%주선호%오추려
脊髓损伤%人脐带间充质干细胞%布洛芬%细胞移植
脊髓損傷%人臍帶間充質榦細胞%佈洛芬%細胞移植
척수손상%인제대간충질간세포%포락분%세포이식
Spinal cord injury%Human umbilical cord mesenchymal stem cell%Ibuprofen%Cell transplantation
目的 观察人脐带间充质干细胞(hUCMSCs)联合布洛芬移植治疗大鼠脊髓损伤的效果,探讨布洛芬对hUCMSCs存活和分化的影响.方法 用Impactor ModelⅡ打击器制作80例大鼠脊髓胸10(T10)损伤模型,随机分为DMEM实验对照组(A组)、布洛芬注射组(B组)、单纯移植hUCMSCs组(C组)、联合治疗组(D组),对损伤后2个月内的实验大鼠进行行为学评分(BBB评分),评估后肢运动功能恢复,结合人细胞核抗体(MAB1281)和神经丝蛋白(NF-200)免疫组织化学染色分析细胞存活和分化.结果 行为学评分D组(14.90±2.45)分,明显高于B组(10.56±2.32)分、C组(11.90±1.89)分,差异有统计学意义(P＜0.05).术后8周观察到D组的hUCMSCs存活数量多于其他治疗组；NF-200染色面积D组(0.28±0.13)高于B组(0.11±0.04)、C组(0.18 ±0.06),差异有统计学意义(P＜0.05).结论 布洛芬可以促进hUCMSCs在损伤部位存活并使其易于向神经样细胞分化,两者联合移植可以明显促进损伤后轴突再生和脊髓功能恢复.
目的 觀察人臍帶間充質榦細胞(hUCMSCs)聯閤佈洛芬移植治療大鼠脊髓損傷的效果,探討佈洛芬對hUCMSCs存活和分化的影響.方法 用Impactor ModelⅡ打擊器製作80例大鼠脊髓胸10(T10)損傷模型,隨機分為DMEM實驗對照組(A組)、佈洛芬註射組(B組)、單純移植hUCMSCs組(C組)、聯閤治療組(D組),對損傷後2箇月內的實驗大鼠進行行為學評分(BBB評分),評估後肢運動功能恢複,結閤人細胞覈抗體(MAB1281)和神經絲蛋白(NF-200)免疫組織化學染色分析細胞存活和分化.結果 行為學評分D組(14.90±2.45)分,明顯高于B組(10.56±2.32)分、C組(11.90±1.89)分,差異有統計學意義(P＜0.05).術後8週觀察到D組的hUCMSCs存活數量多于其他治療組；NF-200染色麵積D組(0.28±0.13)高于B組(0.11±0.04)、C組(0.18 ±0.06),差異有統計學意義(P＜0.05).結論 佈洛芬可以促進hUCMSCs在損傷部位存活併使其易于嚮神經樣細胞分化,兩者聯閤移植可以明顯促進損傷後軸突再生和脊髓功能恢複.
목적 관찰인제대간충질간세포(hUCMSCs)연합포락분이식치료대서척수손상적효과,탐토포락분대hUCMSCs존활화분화적영향.방법 용Impactor ModelⅡ타격기제작80례대서척수흉10(T10)손상모형,수궤분위DMEM실험대조조(A조)、포락분주사조(B조)、단순이식hUCMSCs조(C조)、연합치료조(D조),대손상후2개월내적실험대서진행행위학평분(BBB평분),평고후지운동공능회복,결합인세포핵항체(MAB1281)화신경사단백(NF-200)면역조직화학염색분석세포존활화분화.결과 행위학평분D조(14.90±2.45)분,명현고우B조(10.56±2.32)분、C조(11.90±1.89)분,차이유통계학의의(P＜0.05).술후8주관찰도D조적hUCMSCs존활수량다우기타치료조；NF-200염색면적D조(0.28±0.13)고우B조(0.11±0.04)、C조(0.18 ±0.06),차이유통계학의의(P＜0.05).결론 포락분가이촉진hUCMSCs재손상부위존활병사기역우향신경양세포분화,량자연합이식가이명현촉진손상후축돌재생화척수공능회복.
Objective To investigate the effects of ibuprofen combined with transplantation of human umbilical cord mesenchymal stem cells (hUCMSCs) in rats with spinal cord injury,and explore the influence of ibuprofen on the survival and differentiation of hUCMSCs.Methods Eighty SCI models were made by Impactor Model Ⅱ at T10,and were randomized into 4 groups:DMEM control (group A),Ibuprofen group (group B),hUCMSCs group (group C),co-transplantation (group D).The basso beattie bresnahan (BBB) score was practised to evaluate functional recovery of rat's hind limbs,and immunohistochemistry was used to observe the survival and differentiation of hUCMSCs.Results Functional recovery was more advanced in group D (14.90 ±2.45) points than in group B (10.56 ±2.32) points or group C (11.90 ± 1.89) points according to The BBB scores (P ＜ 0.05).Immunohistochemical staining showed the number of surviving hUCMSCs was more in group than other group,and the NF-200 positive area in cotransplantation at 8th week after transplantation was 0.28 ± 0.13 in group D,greater than in group B (0.11 ± 0.04) or group C (0.18 ± 0.06) (P ＜ 0.05).Conclusion Ibuprofen can promote the survival and differentiation of the transplanted hUCMSCs.Co-transplantation of hUCMSCs and ibuprofen may significantly promote axonal regeneration and functional recovery after SCI.
